2gal
From Proteopedia
(New page: 200px<br /> <applet load="2gal" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gal, resolution 2.00Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:2gal.gif|left|200px]]<br /> | + | [[Image:2gal.gif|left|200px]]<br /><applet load="2gal" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2gal" size=" | + | |
caption="2gal, resolution 2.00Å" /> | caption="2gal, resolution 2.00Å" /> | ||
'''CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH GALACTOSE'''<br /> | '''CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH GALACTOSE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Knowledge about carbohydrate recognition domains of galectins, formerly | + | Knowledge about carbohydrate recognition domains of galectins, formerly known as S-type animal lectins, is important in understanding their role(s) in cell-cell interactions. Here we report the crystal structure of human galectin-7 (hGal-7), in free form and in the presence of galactose, galactosamine, lactose, and N-acetyl-lactosamine at high resolution. This is the first structure of a galectin determined in both free and carbohydrate-bound forms. The structure shows a fold similar to that of the prototype galectins -1 and -2, but has greater similarity to a related galectin molecule, Gal-10. Even though the carbohydrate-binding residues are conserved, there are significant changes in this pocket due to shortening of a loop structure. The monomeric hGal-7 molecule exists as a dimer in the crystals, but adopts a packing arrangement considerably different from that of Gal-1 and Gal-2, which has implications for carbohydrate recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 2GAL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GAL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Acharya, K | + | [[Category: Acharya, K R.]] |
| - | [[Category: Leonidas, D | + | [[Category: Leonidas, D D.]] |
[[Category: GAL]] | [[Category: GAL]] | ||
[[Category: carbohydrate binding]] | [[Category: carbohydrate binding]] | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:48 2008'' |
Revision as of 15:29, 21 February 2008
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CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH GALACTOSE
Overview
Knowledge about carbohydrate recognition domains of galectins, formerly known as S-type animal lectins, is important in understanding their role(s) in cell-cell interactions. Here we report the crystal structure of human galectin-7 (hGal-7), in free form and in the presence of galactose, galactosamine, lactose, and N-acetyl-lactosamine at high resolution. This is the first structure of a galectin determined in both free and carbohydrate-bound forms. The structure shows a fold similar to that of the prototype galectins -1 and -2, but has greater similarity to a related galectin molecule, Gal-10. Even though the carbohydrate-binding residues are conserved, there are significant changes in this pocket due to shortening of a loop structure. The monomeric hGal-7 molecule exists as a dimer in the crystals, but adopts a packing arrangement considerably different from that of Gal-1 and Gal-2, which has implications for carbohydrate recognition.
About this Structure
2GAL is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for the recognition of carbohydrates by human galectin-7., Leonidas DD, Vatzaki EH, Vorum H, Celis JE, Madsen P, Acharya KR, Biochemistry. 1998 Oct 6;37(40):13930-40. PMID:9760227
Page seeded by OCA on Thu Feb 21 17:29:48 2008
