2gat
From Proteopedia
(New page: 200px<br /><applet load="2gat" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gat" /> '''SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN ...) |
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| - | [[Image:2gat.gif|left|200px]]<br /><applet load="2gat" size=" | + | [[Image:2gat.gif|left|200px]]<br /><applet load="2gat" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gat" /> | caption="2gat" /> | ||
'''SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF CHICKEN GATA-1 BOUND TO DNA, NMR, REGULARIZED MEAN STRUCTURE'''<br /> | '''SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF CHICKEN GATA-1 BOUND TO DNA, NMR, REGULARIZED MEAN STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Anisotropy of the molecular magnetic susceptibility gives rise to a small | + | Anisotropy of the molecular magnetic susceptibility gives rise to a small degree of alignment. The resulting residual dipolar couplings, which can now be measured with the advent of higher magnetic fields in NMR, contain information on the orientation of the internuclear vectors relative to the molecular magnetic susceptibility tensor, thereby providing information on long range order that is not accessible by any of the solution NMR parameters currently used in structure determination. Thus, the dipolar couplings constitute unique and powerful restraints in determining the structures of magnetically oriented macromolecules in solution. The method is demonstrated on a complex of the DNA-binding domain of the transcription factor GATA-1 with a 16 base pair oligodeoxyribonucleotide. |
==About this Structure== | ==About this Structure== | ||
| - | 2GAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Clore, G | + | [[Category: Clore, G M.]] |
| - | [[Category: Gronenborn, A | + | [[Category: Gronenborn, A M.]] |
| - | [[Category: Omichinski, J | + | [[Category: Omichinski, J G.]] |
[[Category: Starich, M.]] | [[Category: Starich, M.]] | ||
[[Category: Tjandra, N.]] | [[Category: Tjandra, N.]] | ||
| Line 24: | Line 24: | ||
[[Category: zinc binding domain]] | [[Category: zinc binding domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:56 2008'' |
Revision as of 15:29, 21 February 2008
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SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF CHICKEN GATA-1 BOUND TO DNA, NMR, REGULARIZED MEAN STRUCTURE
Overview
Anisotropy of the molecular magnetic susceptibility gives rise to a small degree of alignment. The resulting residual dipolar couplings, which can now be measured with the advent of higher magnetic fields in NMR, contain information on the orientation of the internuclear vectors relative to the molecular magnetic susceptibility tensor, thereby providing information on long range order that is not accessible by any of the solution NMR parameters currently used in structure determination. Thus, the dipolar couplings constitute unique and powerful restraints in determining the structures of magnetically oriented macromolecules in solution. The method is demonstrated on a complex of the DNA-binding domain of the transcription factor GATA-1 with a 16 base pair oligodeoxyribonucleotide.
About this Structure
2GAT is a Single protein structure of sequence from Gallus gallus with as ligand. Full crystallographic information is available from OCA.
Reference
Use of dipolar 1H-15N and 1H-13C couplings in the structure determination of magnetically oriented macromolecules in solution., Tjandra N, Omichinski JG, Gronenborn AM, Clore GM, Bax A, Nat Struct Biol. 1997 Sep;4(9):732-8. PMID:9303001
Page seeded by OCA on Thu Feb 21 17:29:56 2008
