2gcs
From Proteopedia
(New page: 200px<br /><applet load="2gcs" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gcs, resolution 2.10Å" /> '''Pre-cleavage state o...) |
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==Overview== | ==Overview== | ||
| - | The glmS ribozyme is the only natural catalytic RNA known to require a | + | The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis. |
==About this Structure== | ==About this Structure== | ||
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Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990543 16990543] | Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990543 16990543] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amare, A | + | [[Category: Amare, A R.Ferre-D.]] |
| - | [[Category: Klein, D | + | [[Category: Klein, D J.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: rna; ribozyme; pseudoknot; helix]] | [[Category: rna; ribozyme; pseudoknot; helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:30:27 2008'' |
Revision as of 15:30, 21 February 2008
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Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
Overview
The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
About this Structure
2GCS is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543
Page seeded by OCA on Thu Feb 21 17:30:27 2008
