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2gis

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(New page: 200px<br /><applet load="2gis" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gis, resolution 2.90&Aring;" /> '''Structure of the S-a...)
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==Overview==
==Overview==
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Riboswitches are cis-acting genetic regulatory elements found in the, 5'-untranslated regions of messenger RNAs that control gene expression, through their ability to bind small molecule metabolites directly., Regulation occurs through the interplay of two domains of the RNA: an, aptamer domain that responds to intracellular metabolite concentrations, and an expression platform that uses two mutually exclusive secondary, structures to direct a decision-making process. In Gram-positive bacteria, such as Bacillus species, riboswitches control the expression of more than, 2% of all genes through their ability to respond to a diverse set of, metabolites including amino acids, nucleobases and protein cofactors. Here, we report the 2.9-angstroms resolution crystal structure of an, S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter, tengcongensis complexed with S-adenosylmethionine, an RNA element that, controls the expression of several genes involved in sulphur and, methionine metabolism. This RNA folds into a complex three-dimensional, architecture that recognizes almost every functional group of the ligand, through a combination of direct and indirect readout mechanisms. Ligand, binding induces the formation of a series of tertiary interactions with, one of the helices, serving as a communication link between the aptamer, and expression platform domains.
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Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.
==About this Structure==
==About this Structure==
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Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16810258 16810258]
Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16810258 16810258]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Batey, R.T.]]
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[[Category: Batey, R T.]]
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[[Category: Montange, R.K.]]
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[[Category: Montange, R K.]]
[[Category: IRI]]
[[Category: IRI]]
[[Category: MG]]
[[Category: MG]]
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[[Category: sam]]
[[Category: sam]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:00:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:04 2008''

Revision as of 15:32, 21 February 2008

Image:2gis.gif

2gis, resolution 2.90Å

Drag the structure with the mouse to rotate

Structure of the S-adenosylmethionine riboswitch mRNA regulatory element

Overview

Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.

About this Structure

2GIS is a Protein complex structure of sequences from [1] with , and as ligands. Full crystallographic information is available from OCA.

Reference

Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:16810258

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