2gim

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(New page: 200px<br /><applet load="2gim" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gim, resolution 1.60&Aring;" /> '''1.6 Angstrom structu...)
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[[Image:2gim.gif|left|200px]]<br /><applet load="2gim" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:2gim.gif|left|200px]]<br /><applet load="2gim" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2gim, resolution 1.60&Aring;" />
caption="2gim, resolution 1.60&Aring;" />
'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''<br />
'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''<br />
==Overview==
==Overview==
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Plastocyanin from the cyanobacterium Anabaena variabilis was, heterologously produced in Escherichia coli and purified. Plate-like, crystals were obtained by crystallization in 1.15 M trisodium citrate and, 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized, protein was solved to a resolution of 1.6 Angstrom using plastocyanin from, Phormidium laminosum as a search model. Two molecules were found in the, asymmetric unit. The electrostatic surface of the basic protein showed a, large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged, patches. The copper ion was found to be relatively mobile and there were, two distinct conformations of His61.
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Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.
==About this Structure==
==About this Structure==
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2GIM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis Anabaena variabilis] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA].
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2GIM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis Anabaena variabilis] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA].
==Reference==
==Reference==
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[[Category: Anabaena variabilis]]
[[Category: Anabaena variabilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Christensen, H.E.M.]]
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[[Category: Christensen, H E.M.]]
[[Category: Harris, P.]]
[[Category: Harris, P.]]
[[Category: Schmidt, L.]]
[[Category: Schmidt, L.]]
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[[Category: helix]]
[[Category: helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:13:29 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:02 2008''

Revision as of 15:32, 21 February 2008

Image:2gim.gif

2gim, resolution 1.60Å

Drag the structure with the mouse to rotate

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

Overview

Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.

About this Structure

2GIM is a Single protein structure of sequence from Anabaena variabilis with as ligand. Full crystallographic information is available from OCA.

Reference

Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103

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