2gjd
From Proteopedia
(New page: 200px<br /><applet load="2gjd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gjd, resolution 1.75Å" /> '''Distinct functional ...) |
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| - | [[Image:2gjd.gif|left|200px]]<br /><applet load="2gjd" size=" | + | [[Image:2gjd.gif|left|200px]]<br /><applet load="2gjd" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gjd, resolution 1.75Å" /> | caption="2gjd, resolution 1.75Å" /> | ||
'''Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress'''<br /> | '''Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Covalent modification with SUMO alters protein function, intracellular | + | Covalent modification with SUMO alters protein function, intracellular localization, or protein-protein interactions. Target recognition is determined, in part, by the SUMO E2 enzyme, Ubc9, while Siz/Pias E3 ligases may facilitate select interactions by acting as substrate adaptors. A yeast conditional Ubc9P(123)L mutant was viable at 36 degrees C yet exhibited enhanced sensitivity to DNA damage. To define functional domains in Ubc9 that dictate cellular responses to genotoxic stress versus those necessary for cell viability, a 1.75-A structure of yeast Ubc9 that demonstrated considerable conservation of backbone architecture with human Ubc9 was solved. Nevertheless, differences in side chain geometry/charge guided the design of human/yeast chimeras, where swapping domains implicated in (i) binding residues within substrates that flank canonical SUMOylation sites, (ii) interactions with the RanBP2 E3 ligase, and (iii) binding of the heterodimeric E1 and SUMO had distinct effects on cell growth and resistance to DNA-damaging agents. Our findings establish a functional interaction between N-terminal and substrate-binding domains of Ubc9 and distinguish the activities of E3 ligases Siz1 and Siz2 in regulating cellular responses to genotoxic stress. |
==About this Structure== | ==About this Structure== | ||
| - | 2GJD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http:// | + | 2GJD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ubiquitin--protein ligase]] | [[Category: Ubiquitin--protein ligase]] | ||
| - | [[Category: Bjornsti, M | + | [[Category: Bjornsti, M A.]] |
| - | [[Category: Duda, D | + | [[Category: Duda, D M.]] |
| - | [[Category: Lancaster, C | + | [[Category: Lancaster, C S.]] |
| - | [[Category: Schulman, B | + | [[Category: Schulman, B A.]] |
| - | [[Category: Waardenburg, R | + | [[Category: Waardenburg, R C.van.]] |
[[Category: crystallography]] | [[Category: crystallography]] | ||
[[Category: e2]] | [[Category: e2]] | ||
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[[Category: ubc9p]] | [[Category: ubc9p]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:16 2008'' |
Revision as of 15:32, 21 February 2008
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Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress
Overview
Covalent modification with SUMO alters protein function, intracellular localization, or protein-protein interactions. Target recognition is determined, in part, by the SUMO E2 enzyme, Ubc9, while Siz/Pias E3 ligases may facilitate select interactions by acting as substrate adaptors. A yeast conditional Ubc9P(123)L mutant was viable at 36 degrees C yet exhibited enhanced sensitivity to DNA damage. To define functional domains in Ubc9 that dictate cellular responses to genotoxic stress versus those necessary for cell viability, a 1.75-A structure of yeast Ubc9 that demonstrated considerable conservation of backbone architecture with human Ubc9 was solved. Nevertheless, differences in side chain geometry/charge guided the design of human/yeast chimeras, where swapping domains implicated in (i) binding residues within substrates that flank canonical SUMOylation sites, (ii) interactions with the RanBP2 E3 ligase, and (iii) binding of the heterodimeric E1 and SUMO had distinct effects on cell growth and resistance to DNA-damaging agents. Our findings establish a functional interaction between N-terminal and substrate-binding domains of Ubc9 and distinguish the activities of E3 ligases Siz1 and Siz2 in regulating cellular responses to genotoxic stress.
About this Structure
2GJD is a Single protein structure of sequence from Saccharomyces cerevisiae. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Full crystallographic information is available from OCA.
Reference
Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress., van Waardenburg RC, Duda DM, Lancaster CS, Schulman BA, Bjornsti MA, Mol Cell Biol. 2006 Jul;26(13):4958-69. PMID:16782883
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