2gjw
From Proteopedia
(New page: 200px<br /><applet load="2gjw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gjw, resolution 2.85Å" /> '''RNA Recognition and ...) |
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| - | [[Image:2gjw.gif|left|200px]]<br /><applet load="2gjw" size=" | + | [[Image:2gjw.gif|left|200px]]<br /><applet load="2gjw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gjw, resolution 2.85Å" /> | caption="2gjw, resolution 2.85Å" /> | ||
'''RNA Recognition and Cleavage by an Splicing Endonuclease'''<br /> | '''RNA Recognition and Cleavage by an Splicing Endonuclease'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The RNA splicing endonuclease cleaves two phosphodiester bonds within | + | The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain. |
==About this Structure== | ==About this Structure== | ||
| - | 2GJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] Full crystallographic information is available from [http:// | + | 2GJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: splicing endonuclease af]] | [[Category: splicing endonuclease af]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:26 2008'' |
Revision as of 15:32, 21 February 2008
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RNA Recognition and Cleavage by an Splicing Endonuclease
Overview
The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.
About this Structure
2GJW is a Single protein structure of sequence from Archaeoglobus fulgidus. Active as tRNA-intron endonuclease, with EC number 3.1.27.9 Full crystallographic information is available from OCA.
Reference
RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:16690865
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