2gml
From Proteopedia
(New page: 200px<br /><applet load="2gml" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gml, resolution 2.6Å" /> '''Crystal Structure of ...) |
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| - | [[Image:2gml.gif|left|200px]]<br /><applet load="2gml" size=" | + | [[Image:2gml.gif|left|200px]]<br /><applet load="2gml" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gml, resolution 2.6Å" /> | caption="2gml, resolution 2.6Å" /> | ||
'''Crystal Structure of Catalytic Domain of E.coli RluF'''<br /> | '''Crystal Structure of Catalytic Domain of E.coli RluF'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pseudouridine synthases catalyze the isomerization of uridine to | + | Pseudouridine synthases catalyze the isomerization of uridine to pseudouridine (Psi) in rRNA and tRNA. The pseudouridine synthase RluF from Escherichia coli (E.C. 4.2.1.70) modifies U2604 in 23S rRNA, and belongs to a large family of pseudouridine synthases present in all kingdoms of life. Here we report the domain architecture and crystal structure of the catalytic domain of E.coli RluF at 2.6A resolution. Limited proteolysis, mass spectrometry and N-terminal sequencing indicate that RluF has a distinct domain architecture, with the catalytic domain flanked at the N and C termini by additional domains connected to it by flexible linkers. The structure of the catalytic domain of RluF is similar to those of RsuA and TruB. RluF is a member of the RsuA sequence family of Psi-synthases, along with RluB and RluE. Structural comparison of RluF with its closest structural homologues, RsuA and TruB, suggests possible functional roles for the N-terminal and C-terminal domains of RluF. |
==About this Structure== | ==About this Structure== | ||
| - | 2GML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2GML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GML OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna modifying enzyme]] | [[Category: rna modifying enzyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:10 2008'' |
Revision as of 15:33, 21 February 2008
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Crystal Structure of Catalytic Domain of E.coli RluF
Overview
Pseudouridine synthases catalyze the isomerization of uridine to pseudouridine (Psi) in rRNA and tRNA. The pseudouridine synthase RluF from Escherichia coli (E.C. 4.2.1.70) modifies U2604 in 23S rRNA, and belongs to a large family of pseudouridine synthases present in all kingdoms of life. Here we report the domain architecture and crystal structure of the catalytic domain of E.coli RluF at 2.6A resolution. Limited proteolysis, mass spectrometry and N-terminal sequencing indicate that RluF has a distinct domain architecture, with the catalytic domain flanked at the N and C termini by additional domains connected to it by flexible linkers. The structure of the catalytic domain of RluF is similar to those of RsuA and TruB. RluF is a member of the RsuA sequence family of Psi-synthases, along with RluB and RluE. Structural comparison of RluF with its closest structural homologues, RsuA and TruB, suggests possible functional roles for the N-terminal and C-terminal domains of RluF.
About this Structure
2GML is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Domain organization and crystal structure of the catalytic domain of E.coli RluF, a pseudouridine synthase that acts on 23S rRNA., Sunita S, Zhenxing H, Swaathi J, Cygler M, Matte A, Sivaraman J, J Mol Biol. 2006 Jun 16;359(4):998-1009. Epub 2006 Apr 25. PMID:16712869
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