2goz
From Proteopedia
(New page: 200px<br /><applet load="2goz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2goz, resolution 2.20Å" /> '''The 2.2 A structure ...) |
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==Overview== | ==Overview== | ||
| - | Minimal hammerhead ribozymes have been characterized extensively by static | + | Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerhead ribozyme that permits us to explain the structural basis for its 1000-fold catalytic enhancement. The full-length hammerhead structure reveals how tertiary interactions occurring remotely from the active site prime this ribozyme for catalysis. G-12 and G-8 are positioned consistent with their previously suggested roles in acid-base catalysis, the nucleophile is aligned with a scissile phosphate positioned proximal to the A-9 phosphate, and previously unexplained roles of other conserved nucleotides become apparent within the context of a distinctly new fold that nonetheless accommodates the previous structural studies. These interactions permit us to explain the previously irreconcilable sets of experimental results in a unified, consistent, and unambiguous manner. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Martick, M.]] | [[Category: Martick, M.]] | ||
| - | [[Category: Scott, W | + | [[Category: Scott, W G.]] |
[[Category: a-form helix]] | [[Category: a-form helix]] | ||
[[Category: catalytic rna]] | [[Category: catalytic rna]] | ||
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[[Category: uridine turn]] | [[Category: uridine turn]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:50 2008'' |
Revision as of 15:33, 21 February 2008
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The 2.2 A structure of a full-length catalytically active hammerhead ribozyme
Overview
Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerhead ribozyme that permits us to explain the structural basis for its 1000-fold catalytic enhancement. The full-length hammerhead structure reveals how tertiary interactions occurring remotely from the active site prime this ribozyme for catalysis. G-12 and G-8 are positioned consistent with their previously suggested roles in acid-base catalysis, the nucleophile is aligned with a scissile phosphate positioned proximal to the A-9 phosphate, and previously unexplained roles of other conserved nucleotides become apparent within the context of a distinctly new fold that nonetheless accommodates the previous structural studies. These interactions permit us to explain the previously irreconcilable sets of experimental results in a unified, consistent, and unambiguous manner.
About this Structure
2GOZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Tertiary contacts distant from the active site prime a ribozyme for catalysis., Martick M, Scott WG, Cell. 2006 Jul 28;126(2):309-20. Epub 2006 Jul 20. PMID:16859740
Page seeded by OCA on Thu Feb 21 17:33:50 2008
