2grx
From Proteopedia
(New page: 200px<br /><applet load="2grx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2grx, resolution 3.300Å" /> '''Crystal structure o...) |
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| - | [[Image:2grx.gif|left|200px]]<br /><applet load="2grx" size=" | + | [[Image:2grx.gif|left|200px]]<br /><applet load="2grx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2grx, resolution 3.300Å" /> | caption="2grx, resolution 3.300Å" /> | ||
'''Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome'''<br /> | '''Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The cytoplasmic membrane protein TonB spans the periplasm of the | + | The cytoplasmic membrane protein TonB spans the periplasm of the Gram-negative bacterial cell envelope, contacts cognate outer membrane receptors, and facilitates siderophore transport. The outer membrane receptor FhuA from Escherichia coli mediates TonB-dependent import of ferrichrome. We report the 3.3 angstrom resolution crystal structure of the TonB carboxyl-terminal domain in complex with FhuA. TonB contacts stabilize FhuA's amino-terminal residues, including those of the consensus Ton box sequence that form an interprotein beta sheet with TonB through strand exchange. The highly conserved TonB residue arginine-166 is oriented to form multiple contacts with the FhuA cork, the globular domain enclosed by the beta barrel. |
==About this Structure== | ==About this Structure== | ||
| - | 2GRX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4, FTT, DPO, DAO, MYR, EAP and FCI as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2GRX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=FTT:'>FTT</scene>, <scene name='pdbligand=DPO:'>DPO</scene>, <scene name='pdbligand=DAO:'>DAO</scene>, <scene name='pdbligand=MYR:'>MYR</scene>, <scene name='pdbligand=EAP:'>EAP</scene> and <scene name='pdbligand=FCI:'>FCI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GRX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Allaire, M.]] | [[Category: Allaire, M.]] | ||
| - | [[Category: Coulton, J | + | [[Category: Coulton, J W.]] |
| - | [[Category: Pawelek, P | + | [[Category: Pawelek, P D.]] |
[[Category: DAO]] | [[Category: DAO]] | ||
[[Category: DPO]] | [[Category: DPO]] | ||
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[[Category: protein-protein]] | [[Category: protein-protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:34:40 2008'' |
Revision as of 15:34, 21 February 2008
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Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome
Overview
The cytoplasmic membrane protein TonB spans the periplasm of the Gram-negative bacterial cell envelope, contacts cognate outer membrane receptors, and facilitates siderophore transport. The outer membrane receptor FhuA from Escherichia coli mediates TonB-dependent import of ferrichrome. We report the 3.3 angstrom resolution crystal structure of the TonB carboxyl-terminal domain in complex with FhuA. TonB contacts stabilize FhuA's amino-terminal residues, including those of the consensus Ton box sequence that form an interprotein beta sheet with TonB through strand exchange. The highly conserved TonB residue arginine-166 is oriented to form multiple contacts with the FhuA cork, the globular domain enclosed by the beta barrel.
About this Structure
2GRX is a Protein complex structure of sequences from Escherichia coli with , , , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of TonB in complex with FhuA, E. coli outer membrane receptor., Pawelek PD, Croteau N, Ng-Thow-Hing C, Khursigara CM, Moiseeva N, Allaire M, Coulton JW, Science. 2006 Jun 2;312(5778):1399-402. PMID:16741125
Page seeded by OCA on Thu Feb 21 17:34:40 2008
Categories: Escherichia coli | Protein complex | Allaire, M. | Coulton, J W. | Pawelek, P D. | DAO | DPO | EAP | FCI | FTT | MYR | PO4 | Beta barrel | Heterocomplex | Inter-protein beta sheet | Outer membrane | Protein-protein
