This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2gsk
From Proteopedia
(New page: 200px<br /><applet load="2gsk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gsk, resolution 2.10Å" /> '''Structure of the Btu...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2gsk.gif|left|200px]]<br /><applet load="2gsk" size=" | + | [[Image:2gsk.gif|left|200px]]<br /><applet load="2gsk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gsk, resolution 2.10Å" /> | caption="2gsk, resolution 2.10Å" /> | ||
'''Structure of the BtuB:TonB Complex'''<br /> | '''Structure of the BtuB:TonB Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In Gram-negative bacteria, the import of essential micronutrients across | + | In Gram-negative bacteria, the import of essential micronutrients across the outer membrane requires a transporter, an electrochemical gradient of protons across the inner membrane, and an inner membrane protein complex (ExbB, ExbD, TonB) that couples the proton-motive force to the outer membrane transporter. The inner membrane protein TonB binds directly to a conserved region, called the Ton-box, of the transporter. We solved the structure of the cobalamin transporter BtuB in complex with the C-terminal domain of TonB. In contrast to its conformations in the absence of TonB, the Ton-box forms a beta strand that is recruited to the existing beta sheet of TonB, which is consistent with a mechanical pulling model of transport. |
==About this Structure== | ==About this Structure== | ||
| - | 2GSK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA, CNC, LDA, OCT and HEX as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2GSK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=CNC:'>CNC</scene>, <scene name='pdbligand=LDA:'>LDA</scene>, <scene name='pdbligand=OCT:'>OCT</scene> and <scene name='pdbligand=HEX:'>HEX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSK OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Banchs, C | + | [[Category: Banchs, C N.]] |
| - | [[Category: Purdy, M | + | [[Category: Purdy, M P.]] |
| - | [[Category: Shultis, D | + | [[Category: Shultis, D D.]] |
| - | [[Category: Wiener, M | + | [[Category: Wiener, M C.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: CNC]] | [[Category: CNC]] | ||
| Line 27: | Line 27: | ||
[[Category: tonb]] | [[Category: tonb]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:34:52 2008'' |
Revision as of 15:34, 21 February 2008
|
Structure of the BtuB:TonB Complex
Overview
In Gram-negative bacteria, the import of essential micronutrients across the outer membrane requires a transporter, an electrochemical gradient of protons across the inner membrane, and an inner membrane protein complex (ExbB, ExbD, TonB) that couples the proton-motive force to the outer membrane transporter. The inner membrane protein TonB binds directly to a conserved region, called the Ton-box, of the transporter. We solved the structure of the cobalamin transporter BtuB in complex with the C-terminal domain of TonB. In contrast to its conformations in the absence of TonB, the Ton-box forms a beta strand that is recruited to the existing beta sheet of TonB, which is consistent with a mechanical pulling model of transport.
About this Structure
2GSK is a Protein complex structure of sequences from Escherichia coli with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Outer membrane active transport: structure of the BtuB:TonB complex., Shultis DD, Purdy MD, Banchs CN, Wiener MC, Science. 2006 Jun 2;312(5778):1396-9. PMID:16741124
Page seeded by OCA on Thu Feb 21 17:34:52 2008
Categories: Escherichia coli | Protein complex | Banchs, C N. | Purdy, M P. | Shultis, D D. | Wiener, M C. | CA | CNC | HEX | LDA | OCT | Beta-barrel | Membrane protein | Outer-membrane active transport | Tonb
