2gsj
From Proteopedia
(New page: 200px<br /><applet load="2gsj" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gsj, resolution 1.73Å" /> '''cDNA cloning and 1.7...) |
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==Overview== | ==Overview== | ||
| - | Parkia platycephala lectin 2 was purified from Parkia platycephala | + | Parkia platycephala lectin 2 was purified from Parkia platycephala (Leguminosae, Mimosoideae) seeds by affinity chromatography and RP-HPLC. Equilibrium sedimentation and MS showed that Parkia platycephala lectin 2 is a nonglycosylated monomeric protein of molecular mass 29 407+/-15 Da, which contains six cysteine residues engaged in the formation of three intramolecular disulfide bonds. Parkia platycephala lectin 2 agglutinated rabbit erythrocytes, and this activity was specifically inhibited by N-acetylglucosamine. In addition, Parkia platycephala lectin 2 hydrolyzed beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. The full-length amino acid sequence of Parkia platycephala lectin 2, determined by N-terminal sequencing and cDNA cloning, and its three-dimensional structure, established by X-ray crystallography at 1.75 A resolution, showed that Parkia platycephala lectin 2 is homologous to endochitinases of the glycosyl hydrolase family 18, which share the (betaalpha)8 barrel topology harboring the catalytic residues Asp125, Glu127, and Tyr182. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Barettino, D.]] | [[Category: Barettino, D.]] | ||
| - | [[Category: Calvete, J | + | [[Category: Calvete, J J.]] |
| - | [[Category: Castellon, R | + | [[Category: Castellon, R E.R.]] |
| - | [[Category: Cavada, B | + | [[Category: Cavada, B S.]] |
[[Category: Debray, H.]] | [[Category: Debray, H.]] | ||
[[Category: Delatorre, P.]] | [[Category: Delatorre, P.]] | ||
| - | [[Category: Goersch, G | + | [[Category: Goersch, G V.]] |
| - | [[Category: Jr., W | + | [[Category: Jr., W F.de Azevedo.]] |
[[Category: Leroy, Y.]] | [[Category: Leroy, Y.]] | ||
| - | [[Category: Moreno, F | + | [[Category: Moreno, F B.]] |
| - | [[Category: Nagano, C | + | [[Category: Nagano, C S.]] |
| - | [[Category: Nascimento, K | + | [[Category: Nascimento, K S.]] |
| - | [[Category: Pinto, V | + | [[Category: Pinto, V P.]] |
[[Category: Radis-Baptista, G.]] | [[Category: Radis-Baptista, G.]] | ||
| - | [[Category: Rocha, B | + | [[Category: Rocha, B A.da.]] |
| - | [[Category: Sampaio, A | + | [[Category: Sampaio, A H.]] |
[[Category: Sanz, L.]] | [[Category: Sanz, L.]] | ||
| - | [[Category: Souza, E | + | [[Category: Souza, E P.de.]] |
| - | [[Category: Toyama, M | + | [[Category: Toyama, M H.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: chimerolectin]] | [[Category: chimerolectin]] | ||
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[[Category: x-ray crystal structure]] | [[Category: x-ray crystal structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:34:53 2008'' |
Revision as of 15:34, 21 February 2008
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cDNA cloning and 1.75A crystal structure determination of PPL2, a novel chimerolectin from Parkia platycephala seeds exhibiting endochitinolytic activity
Overview
Parkia platycephala lectin 2 was purified from Parkia platycephala (Leguminosae, Mimosoideae) seeds by affinity chromatography and RP-HPLC. Equilibrium sedimentation and MS showed that Parkia platycephala lectin 2 is a nonglycosylated monomeric protein of molecular mass 29 407+/-15 Da, which contains six cysteine residues engaged in the formation of three intramolecular disulfide bonds. Parkia platycephala lectin 2 agglutinated rabbit erythrocytes, and this activity was specifically inhibited by N-acetylglucosamine. In addition, Parkia platycephala lectin 2 hydrolyzed beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. The full-length amino acid sequence of Parkia platycephala lectin 2, determined by N-terminal sequencing and cDNA cloning, and its three-dimensional structure, established by X-ray crystallography at 1.75 A resolution, showed that Parkia platycephala lectin 2 is homologous to endochitinases of the glycosyl hydrolase family 18, which share the (betaalpha)8 barrel topology harboring the catalytic residues Asp125, Glu127, and Tyr182.
About this Structure
2GSJ is a Protein complex structure of sequences from Parkia platycephala with as ligand. Full crystallographic information is available from OCA.
Reference
cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds., Cavada BS, Moreno FB, da Rocha BA, de Azevedo WF Jr, Castellon RE, Goersch GV, Nagano CS, de Souza EP, Nascimento KS, Radis-Baptista G, Delatorre P, Leroy Y, Toyama MH, Pinto VP, Sampaio AH, Barettino D, Debray H, Calvete JJ, Sanz L, FEBS J. 2006 Sep;273(17):3962-74. PMID:16934035
Page seeded by OCA on Thu Feb 21 17:34:53 2008
Categories: Parkia platycephala | Protein complex | Barettino, D. | Calvete, J J. | Castellon, R E.R. | Cavada, B S. | Debray, H. | Delatorre, P. | Goersch, G V. | Jr., W F.de Azevedo. | Leroy, Y. | Moreno, F B. | Nagano, C S. | Nascimento, K S. | Pinto, V P. | Radis-Baptista, G. | Rocha, B A.da. | Sampaio, A H. | Sanz, L. | Souza, E P.de. | Toyama, M H. | SO4 | Chimerolectin | Endochitinase | Equilibrium sedimentation | Glycosyl hydrolase family 18 | Mimosoideae | X-ray crystal structure
