2gsy
From Proteopedia
(New page: 200px<br /><applet load="2gsy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gsy, resolution 2.60Å" /> '''The 2.6A structure o...) |
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| - | [[Image:2gsy.gif|left|200px]]<br /><applet load="2gsy" size=" | + | [[Image:2gsy.gif|left|200px]]<br /><applet load="2gsy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gsy, resolution 2.60Å" /> | caption="2gsy, resolution 2.60Å" /> | ||
'''The 2.6A structure of Infectious Bursal Virus Derived T=1 Particles'''<br /> | '''The 2.6A structure of Infectious Bursal Virus Derived T=1 Particles'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Infectious bursal disease virus (IBDV), a member of the Birnaviridae | + | Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is a double-stranded RNA virus that causes a highly contagious disease in young chickens leading to significant economic losses in the poultry industry. The VP2 protein, the only structural component of the IBDV icosahedral capsid, spontaneously assembles into T=1 subviral particles (SVP) when individually expressed as a chimeric gene. We have determined the crystal structure of the T=1 SVP to 2.60 A resolution. Our results show that the 20 trimeric VP2 clusters forming the T=1 shell are further stabilized by calcium ions located at the threefold icosahedral axes. The structure also reveals a new unexpected domain swapping that mediates interactions between adjacent trimers: a short helical segment located close to the end of the long C-terminal arm of VP2 is projected toward the threefold axis of a neighboring VP2 trimer, leading to a complex network of interactions that increases the stability of the T=1 particles. Analysis of crystal packing shows that the exposed capsid residues, His253 and Thr284, determinants of IBDV virulence and the adaptation of the virus to grow in cell culture, are involved in particle-particle interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 2GSY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Infectious_bursal_disease_virus Infectious bursal disease virus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GSY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Infectious_bursal_disease_virus Infectious bursal disease virus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Abaitua, F.]] | [[Category: Abaitua, F.]] | ||
| - | [[Category: Caston, J | + | [[Category: Caston, J R.]] |
[[Category: Garriga, D.]] | [[Category: Garriga, D.]] | ||
[[Category: Pous, J.]] | [[Category: Pous, J.]] | ||
[[Category: Querol-Audi, J.]] | [[Category: Querol-Audi, J.]] | ||
| - | [[Category: Rodriguez, J | + | [[Category: Rodriguez, J F.]] |
[[Category: Saugar, I.]] | [[Category: Saugar, I.]] | ||
[[Category: Verdaguer, N.]] | [[Category: Verdaguer, N.]] | ||
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[[Category: virus-like capsid (protein-protein complex)]] | [[Category: virus-like capsid (protein-protein complex)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:35:01 2008'' |
Revision as of 15:35, 21 February 2008
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The 2.6A structure of Infectious Bursal Virus Derived T=1 Particles
Overview
Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is a double-stranded RNA virus that causes a highly contagious disease in young chickens leading to significant economic losses in the poultry industry. The VP2 protein, the only structural component of the IBDV icosahedral capsid, spontaneously assembles into T=1 subviral particles (SVP) when individually expressed as a chimeric gene. We have determined the crystal structure of the T=1 SVP to 2.60 A resolution. Our results show that the 20 trimeric VP2 clusters forming the T=1 shell are further stabilized by calcium ions located at the threefold icosahedral axes. The structure also reveals a new unexpected domain swapping that mediates interactions between adjacent trimers: a short helical segment located close to the end of the long C-terminal arm of VP2 is projected toward the threefold axis of a neighboring VP2 trimer, leading to a complex network of interactions that increases the stability of the T=1 particles. Analysis of crystal packing shows that the exposed capsid residues, His253 and Thr284, determinants of IBDV virulence and the adaptation of the virus to grow in cell culture, are involved in particle-particle interactions.
About this Structure
2GSY is a Single protein structure of sequence from Infectious bursal disease virus with as ligand. Full crystallographic information is available from OCA.
Reference
The 2.6-Angstrom structure of infectious bursal disease virus-derived T=1 particles reveals new stabilizing elements of the virus capsid., Garriga D, Querol-Audi J, Abaitua F, Saugar I, Pous J, Verdaguer N, Caston JR, Rodriguez JF, J Virol. 2006 Jul;80(14):6895-905. PMID:16809295
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