2gsq
From Proteopedia
(New page: 200px<br /><applet load="2gsq" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gsq, resolution 2.2Å" /> '''GLUTATHIONE S-TRANSFE...) |
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| - | [[Image:2gsq.jpg|left|200px]]<br /><applet load="2gsq" size=" | + | [[Image:2gsq.jpg|left|200px]]<br /><applet load="2gsq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gsq, resolution 2.2Å" /> | caption="2gsq, resolution 2.2Å" /> | ||
'''GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE'''<br /> | '''GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the sigma class glutathione transferase from | + | The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates. |
==About this Structure== | ==About this Structure== | ||
| - | 2GSQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ommastrephes_sloani Ommastrephes sloani] with SO4 and GBI as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http:// | + | 2GSQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ommastrephes_sloani Ommastrephes sloani] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GBI:'>GBI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ommastrephes sloani]] | [[Category: Ommastrephes sloani]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Armstrong, R | + | [[Category: Armstrong, R N.]] |
| - | [[Category: Gilliland, G | + | [[Category: Gilliland, G L.]] |
[[Category: Ji, X.]] | [[Category: Ji, X.]] | ||
[[Category: GBI]] | [[Category: GBI]] | ||
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[[Category: squid digestive gland]] | [[Category: squid digestive gland]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:35:11 2008'' |
Revision as of 15:35, 21 February 2008
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GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE
Overview
The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates.
About this Structure
2GSQ is a Single protein structure of sequence from Ommastrephes sloani with and as ligands. Active as Glutathione transferase, with EC number 2.5.1.18 Full crystallographic information is available from OCA.
Reference
Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography., Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL, Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:8710848
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