2gtl
From Proteopedia
(New page: 200px<br /> <applet load="2gtl" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gtl, resolution 3.500Å" /> '''Lumbricus Erythroc...) |
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| - | [[Image:2gtl.gif|left|200px]]<br /> | + | [[Image:2gtl.gif|left|200px]]<br /><applet load="2gtl" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2gtl" size=" | + | |
caption="2gtl, resolution 3.500Å" /> | caption="2gtl, resolution 3.500Å" /> | ||
'''Lumbricus Erythrocruorin at 3.5A resolution'''<br /> | '''Lumbricus Erythrocruorin at 3.5A resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Annelid erythrocruorins are highly cooperative extracellular respiratory | + | Annelid erythrocruorins are highly cooperative extracellular respiratory proteins with molecular masses on the order of 3.6 million Daltons. We report here the 3.5 A crystal structure of erythrocruorin from the earthworm Lumbricus terrestris. This structure reveals details of symmetrical and quasi-symmetrical interactions that dictate the self-limited assembly of 144 hemoglobin and 36 linker subunits. The linker subunits assemble into a core complex with D(6) symmetry onto which 12 hemoglobin dodecamers bind to form the entire complex. Although the three unique linker subunits share structural similarity, their interactions with each other and the hemoglobin subunits display striking diversity. The observed diversity includes design features that have been incorporated into the linker subunits and may be critical for efficient assembly of large quantities of this complex respiratory protein. |
==About this Structure== | ==About this Structure== | ||
| - | 2GTL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris] with CA, ZN, CMO and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2GTL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CMO:'>CMO</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bhyravbhatla, B.]] | [[Category: Bhyravbhatla, B.]] | ||
| - | [[Category: Jr., W | + | [[Category: Jr., W E.Royer.]] |
| - | [[Category: Knapp, J | + | [[Category: Knapp, J E.]] |
[[Category: Sharma, H.]] | [[Category: Sharma, H.]] | ||
[[Category: Strand, K.]] | [[Category: Strand, K.]] | ||
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[[Category: triple stranded helical coils]] | [[Category: triple stranded helical coils]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:35:13 2008'' |
Revision as of 15:35, 21 February 2008
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Lumbricus Erythrocruorin at 3.5A resolution
Overview
Annelid erythrocruorins are highly cooperative extracellular respiratory proteins with molecular masses on the order of 3.6 million Daltons. We report here the 3.5 A crystal structure of erythrocruorin from the earthworm Lumbricus terrestris. This structure reveals details of symmetrical and quasi-symmetrical interactions that dictate the self-limited assembly of 144 hemoglobin and 36 linker subunits. The linker subunits assemble into a core complex with D(6) symmetry onto which 12 hemoglobin dodecamers bind to form the entire complex. Although the three unique linker subunits share structural similarity, their interactions with each other and the hemoglobin subunits display striking diversity. The observed diversity includes design features that have been incorporated into the linker subunits and may be critical for efficient assembly of large quantities of this complex respiratory protein.
About this Structure
2GTL is a Protein complex structure of sequences from Lumbricus terrestris with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Lumbricus erythrocruorin at 3.5 A resolution: architecture of a megadalton respiratory complex., Royer WE Jr, Sharma H, Strand K, Knapp JE, Bhyravbhatla B, Structure. 2006 Jul;14(7):1167-77. PMID:16843898
Page seeded by OCA on Thu Feb 21 17:35:13 2008
