2gv4
From Proteopedia
(New page: 200px<br /><applet load="2gv4" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gv4" /> '''Solution structure of the poliovirus 3'-UTR ...) |
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==Overview== | ==Overview== | ||
| - | The previously described NMR structure of a 5'-CU-3'/5'-UU-3' motif, which | + | The previously described NMR structure of a 5'-CU-3'/5'-UU-3' motif, which is highly conserved within the 3'-UTR Y-stem of poliovirus-like enteroviruses, revealed striking regularities of the local helix geometry, thus retaining the pseudo-twofold symmetry of the RNA helix. A mutant virus with both pyrimidine base pairs changed into Watson-Crick replicated as wild type, indicating the functional importance of this symmetry relation in viral RNA replication. Here we investigated the effect of changing only one of the two pyrimidine base pairs to Watson-Crick. We determined the NMR structures of two Y-stem variants: one containing the 5'-CU-3'/5'-AU-3' motif, which has been found in wild-type virus isolates as well, and the other containing a 5'-CU-3'/5'-UG-3' motif, which is not present in any enterovirus sequenced to date. Both structures show single pyrimidine mismatches with intercalated bases. In the 5'-CU-3'/5'-AU-3' motif a C-U Watson-Crick-type base pair is formed that retains the pseudo-twofold symmetry, while in the 5'-CU-3'/5'-UG-3' motif a single asymmetric U-U mismatch breaks the twofold symmetry. Surprisingly, for the nonnatural variant no effect of the single base-pair replacement was observed on polioviral RNA replication using an in vitro replicon assay. |
==About this Structure== | ==About this Structure== | ||
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Breaking pseudo-twofold symmetry in the poliovirus 3'-UTR Y-stem by restoring Watson-Crick base pairs., Zoll J, Tessari M, Van Kuppeveld FJ, Melchers WJ, Heus HA, RNA. 2007 May;13(5):781-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17449731 17449731] | Breaking pseudo-twofold symmetry in the poliovirus 3'-UTR Y-stem by restoring Watson-Crick base pairs., Zoll J, Tessari M, Van Kuppeveld FJ, Melchers WJ, Heus HA, RNA. 2007 May;13(5):781-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17449731 17449731] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Heus, H | + | [[Category: Heus, H A.]] |
| - | [[Category: Kuppeveld, F | + | [[Category: Kuppeveld, F J.M van.]] |
| - | [[Category: Melchers, W | + | [[Category: Melchers, W J.G.]] |
[[Category: Tessari, M.]] | [[Category: Tessari, M.]] | ||
[[Category: Zoll, J.]] | [[Category: Zoll, J.]] | ||
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[[Category: stem-loop]] | [[Category: stem-loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:35:41 2008'' |
Revision as of 15:35, 21 February 2008
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Solution structure of the poliovirus 3'-UTR Y-stem
Overview
The previously described NMR structure of a 5'-CU-3'/5'-UU-3' motif, which is highly conserved within the 3'-UTR Y-stem of poliovirus-like enteroviruses, revealed striking regularities of the local helix geometry, thus retaining the pseudo-twofold symmetry of the RNA helix. A mutant virus with both pyrimidine base pairs changed into Watson-Crick replicated as wild type, indicating the functional importance of this symmetry relation in viral RNA replication. Here we investigated the effect of changing only one of the two pyrimidine base pairs to Watson-Crick. We determined the NMR structures of two Y-stem variants: one containing the 5'-CU-3'/5'-AU-3' motif, which has been found in wild-type virus isolates as well, and the other containing a 5'-CU-3'/5'-UG-3' motif, which is not present in any enterovirus sequenced to date. Both structures show single pyrimidine mismatches with intercalated bases. In the 5'-CU-3'/5'-AU-3' motif a C-U Watson-Crick-type base pair is formed that retains the pseudo-twofold symmetry, while in the 5'-CU-3'/5'-UG-3' motif a single asymmetric U-U mismatch breaks the twofold symmetry. Surprisingly, for the nonnatural variant no effect of the single base-pair replacement was observed on polioviral RNA replication using an in vitro replicon assay.
About this Structure
2GV4 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Breaking pseudo-twofold symmetry in the poliovirus 3'-UTR Y-stem by restoring Watson-Crick base pairs., Zoll J, Tessari M, Van Kuppeveld FJ, Melchers WJ, Heus HA, RNA. 2007 May;13(5):781-92. PMID:17449731
Page seeded by OCA on Thu Feb 21 17:35:41 2008
