2gwm
From Proteopedia
(New page: 200px<br /><applet load="2gwm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gwm, resolution 1.500Å" /> '''Crystal structure o...) |
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| - | [[Image:2gwm.gif|left|200px]]<br /><applet load="2gwm" size=" | + | [[Image:2gwm.gif|left|200px]]<br /><applet load="2gwm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2gwm, resolution 1.500Å" /> | caption="2gwm, resolution 1.500Å" /> | ||
'''Crystal structure of the Salmonella SpvB ATR Domain'''<br /> | '''Crystal structure of the Salmonella SpvB ATR Domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB | + | Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts. |
==About this Structure== | ==About this Structure== | ||
| - | 2GWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Margarit, S | + | [[Category: Margarit, S M.]] |
| - | [[Category: Stebbins, C | + | [[Category: Stebbins, C E.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: adp-ribosyltransferase]] | [[Category: adp-ribosyltransferase]] | ||
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[[Category: spvb]] | [[Category: spvb]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:08 2008'' |
Revision as of 15:36, 21 February 2008
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Crystal structure of the Salmonella SpvB ATR Domain
Overview
Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.
About this Structure
2GWM is a Single protein structure of sequence from Salmonella typhimurium with as ligand. Full crystallographic information is available from OCA.
Reference
A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:16905096
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