This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2gyd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2gyd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gyd, resolution 1.720&Aring;" /> '''Complex of equine a...)
Line 1: Line 1:
-
[[Image:2gyd.gif|left|200px]]<br /><applet load="2gyd" size="450" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2gyd.gif|left|200px]]<br /><applet load="2gyd" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2gyd, resolution 1.720&Aring;" />
caption="2gyd, resolution 1.720&Aring;" />
'''Complex of equine apoferritin with the H-diaziflurane photolabeling reagent'''<br />
'''Complex of equine apoferritin with the H-diaziflurane photolabeling reagent'''<br />
==Overview==
==Overview==
-
The difficulty in obtaining binding target and site information for, low-affinity drugs, like the inhaled anesthetics, has limited, identification of their molecular effectors. Because such information can, be provided by photoactive analogues, we designed, synthesized, and, characterized a novel diazirnyl haloether that closely mimics isoflurane, the most widely used clinical general anesthetic. This compound, H-diaziflurane, is a nontoxic, potent anesthetic that potentiates, GABA-gated ion channels in primary cultures of hippocampal neurons., Calorimetric and structural characterizations show that H-diaziflurane, binds a model anesthetic host protein with similar energetics as, isoflurane and forms photoadducts with residues lining the isoflurane, binding site. H-diaziflurane will be immediately useful for identifying, targets and sites important for the molecular pharmacology of the inhaled, haloether anesthetics.
+
The difficulty in obtaining binding target and site information for low-affinity drugs, like the inhaled anesthetics, has limited identification of their molecular effectors. Because such information can be provided by photoactive analogues, we designed, synthesized, and characterized a novel diazirnyl haloether that closely mimics isoflurane, the most widely used clinical general anesthetic. This compound, H-diaziflurane, is a nontoxic, potent anesthetic that potentiates GABA-gated ion channels in primary cultures of hippocampal neurons. Calorimetric and structural characterizations show that H-diaziflurane binds a model anesthetic host protein with similar energetics as isoflurane and forms photoadducts with residues lining the isoflurane binding site. H-diaziflurane will be immediately useful for identifying targets and sites important for the molecular pharmacology of the inhaled haloether anesthetics.
==About this Structure==
==About this Structure==
-
2GYD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus] with CD and DFE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GYD OCA].
+
2GYD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus] with <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=DFE:'>DFE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GYD OCA].
==Reference==
==Reference==
Line 14: Line 14:
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Eckenhoff, R.]]
[[Category: Eckenhoff, R.]]
-
[[Category: Loll, P.J.]]
+
[[Category: Loll, P J.]]
-
[[Category: Rossi, M.J.]]
+
[[Category: Rossi, M J.]]
[[Category: CD]]
[[Category: CD]]
[[Category: DFE]]
[[Category: DFE]]
Line 22: Line 22:
[[Category: isoflurane]]
[[Category: isoflurane]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:27:36 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:25 2008''

Revision as of 15:36, 21 February 2008

Image:2gyd.gif

2gyd, resolution 1.720Å

Drag the structure with the mouse to rotate

Complex of equine apoferritin with the H-diaziflurane photolabeling reagent

Overview

The difficulty in obtaining binding target and site information for low-affinity drugs, like the inhaled anesthetics, has limited identification of their molecular effectors. Because such information can be provided by photoactive analogues, we designed, synthesized, and characterized a novel diazirnyl haloether that closely mimics isoflurane, the most widely used clinical general anesthetic. This compound, H-diaziflurane, is a nontoxic, potent anesthetic that potentiates GABA-gated ion channels in primary cultures of hippocampal neurons. Calorimetric and structural characterizations show that H-diaziflurane binds a model anesthetic host protein with similar energetics as isoflurane and forms photoadducts with residues lining the isoflurane binding site. H-diaziflurane will be immediately useful for identifying targets and sites important for the molecular pharmacology of the inhaled haloether anesthetics.

About this Structure

2GYD is a Single protein structure of sequence from Equus caballus with and as ligands. Full crystallographic information is available from OCA.

Reference

Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions., Xi J, Liu R, Rossi MJ, Yang J, Loll PJ, Dailey WP, Eckenhoff RG, ACS Chem Biol. 2006 Jul 21;1(6):377-84. PMID:17163775

Page seeded by OCA on Thu Feb 21 17:36:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gyd"
Personal tools