2h0q
From Proteopedia
(New page: 200px<br /><applet load="2h0q" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h0q, resolution 1.82Å" /> '''Crystal Structure of...) |
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==Overview== | ==Overview== | ||
| - | Precise signaling by the T cell receptor (TCR) is crucial for a proper | + | Precise signaling by the T cell receptor (TCR) is crucial for a proper immune response. To ensure that T cells respond appropriately to antigenic stimuli, TCR signaling pathways are subject to multiple levels of regulation. Sts-1 negatively regulates signaling pathways downstream of the TCR by an unknown mechanism(s). Here, we demonstrate that Sts-1 is a phosphatase that can target the tyrosine kinase Zap-70 among other proteins. The X-ray structure of the Sts-1 C terminus reveals that it has homology to members of the phosphoglycerate mutase/acid phosphatase (PGM/AcP) family of enzymes, with residues known to be important for PGM/AcP catalytic activity conserved in nature and position in Sts-1. Point mutations that impair Sts-1 phosphatase activity in vitro also impair the ability of Sts-1 to regulate TCR signaling in T cells. These observations reveal a PGM/AcP-like enzyme activity involved in the control of antigen receptor signaling. |
==About this Structure== | ==About this Structure== | ||
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[[Category: sts-1]] | [[Category: sts-1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:37:00 2008'' |
Revision as of 15:37, 21 February 2008
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Crystal Structure of the PGM domain of the Suppressor of T-Cell receptor (Sts-1)
Overview
Precise signaling by the T cell receptor (TCR) is crucial for a proper immune response. To ensure that T cells respond appropriately to antigenic stimuli, TCR signaling pathways are subject to multiple levels of regulation. Sts-1 negatively regulates signaling pathways downstream of the TCR by an unknown mechanism(s). Here, we demonstrate that Sts-1 is a phosphatase that can target the tyrosine kinase Zap-70 among other proteins. The X-ray structure of the Sts-1 C terminus reveals that it has homology to members of the phosphoglycerate mutase/acid phosphatase (PGM/AcP) family of enzymes, with residues known to be important for PGM/AcP catalytic activity conserved in nature and position in Sts-1. Point mutations that impair Sts-1 phosphatase activity in vitro also impair the ability of Sts-1 to regulate TCR signaling in T cells. These observations reveal a PGM/AcP-like enzyme activity involved in the control of antigen receptor signaling.
About this Structure
2H0Q is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
A phosphatase activity of Sts-1 contributes to the suppression of TCR signaling., Mikhailik A, Ford B, Keller J, Chen Y, Nassar N, Carpino N, Mol Cell. 2007 Aug 3;27(3):486-97. PMID:17679096
Page seeded by OCA on Thu Feb 21 17:37:00 2008
Categories: Mus musculus | Single protein | Carpino, N. | Ford, B. | Nassar, N. | Pgm | Signaling protein | Sts-1
