This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2h0s
From Proteopedia
(New page: 200px<br /><applet load="2h0s" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h0s, resolution 2.35Å" /> '''Pre-cleavage state o...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The glmS ribozyme is the only natural catalytic RNA known to require a | + | The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis. |
==About this Structure== | ==About this Structure== | ||
| Line 12: | Line 12: | ||
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990543 16990543] | Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990543 16990543] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amare, A | + | [[Category: Amare, A R.Ferre-D.]] |
| - | [[Category: Klein, D | + | [[Category: Klein, D J.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: helix]] | [[Category: helix]] | ||
| Line 20: | Line 20: | ||
[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:37:01 2008'' |
Revision as of 15:37, 21 February 2008
|
Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
Overview
The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
About this Structure
2H0S is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543
Page seeded by OCA on Thu Feb 21 17:37:01 2008
Categories: Single protein | Amare, A R.Ferre-D. | Klein, D J. | MG | Helix | Pseudoknot | Ribozyme | Rna
