2h1m
From Proteopedia
(New page: 200px<br /><applet load="2h1m" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h1m, resolution 2.90Å" /> '''Synthesis, Oxidation...) |
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==Overview== | ==Overview== | ||
| - | We have recently introduced a basic concept for the combined chemical and | + | We have recently introduced a basic concept for the combined chemical and enzymatic preparation of site-specifically modified 2'-methylseleno RNAs which represent useful derivatives for phasing of X-ray crystallographic data during their three-dimensional structure determination. Here, we introduce the first synthesis of an appropriate guanosine phosphoramidite, which complements the thus far established set of 2'-methylseleno-modified uridine, cytidine, and adenosine building blocks for solid-phase synthesis. The novel building block was readily incorporated into RNA. Importantly, it was the 2'-methylseleno-guanosine-labeled RNA that allowed us to reveal the reversible oxidation/reduction behavior of the Se moiety and thus it represents a valuable contribution to the understanding of the action of threo-1,4-dimercapto-2,3-butanediol (DTT) required during solid-phase synthesis, deprotection, and crystallization of selenium-containing RNA. In addition, we investigated 2'-methylseleno RNA with respect to crystallization properties. Our studies revealed that the Se modification significantly increases the range of conditions leading to crystal growth. Moreover, we determined the crystal structures of model RNA helices and showed that the Se modification can affect crystal packing interactions, thus potentially expanding the possibilities for obtaining the best crystal form. |
==About this Structure== | ==About this Structure== | ||
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Synthesis, oxidation behavior, crystallization and structure of 2'-methylseleno guanosine containing RNAs., Moroder H, Kreutz C, Lang K, Serganov A, Micura R, J Am Chem Soc. 2006 Aug 2;128(30):9909-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16866550 16866550] | Synthesis, oxidation behavior, crystallization and structure of 2'-methylseleno guanosine containing RNAs., Moroder H, Kreutz C, Lang K, Serganov A, Micura R, J Am Chem Soc. 2006 Aug 2;128(30):9909-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16866550 16866550] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Serganov, A | + | [[Category: Serganov, A A.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: a-rna structure]] | [[Category: a-rna structure]] | ||
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[[Category: se 2'-methylse]] | [[Category: se 2'-methylse]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:37:16 2008'' |
Revision as of 15:37, 21 February 2008
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Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs
Overview
We have recently introduced a basic concept for the combined chemical and enzymatic preparation of site-specifically modified 2'-methylseleno RNAs which represent useful derivatives for phasing of X-ray crystallographic data during their three-dimensional structure determination. Here, we introduce the first synthesis of an appropriate guanosine phosphoramidite, which complements the thus far established set of 2'-methylseleno-modified uridine, cytidine, and adenosine building blocks for solid-phase synthesis. The novel building block was readily incorporated into RNA. Importantly, it was the 2'-methylseleno-guanosine-labeled RNA that allowed us to reveal the reversible oxidation/reduction behavior of the Se moiety and thus it represents a valuable contribution to the understanding of the action of threo-1,4-dimercapto-2,3-butanediol (DTT) required during solid-phase synthesis, deprotection, and crystallization of selenium-containing RNA. In addition, we investigated 2'-methylseleno RNA with respect to crystallization properties. Our studies revealed that the Se modification significantly increases the range of conditions leading to crystal growth. Moreover, we determined the crystal structures of model RNA helices and showed that the Se modification can affect crystal packing interactions, thus potentially expanding the possibilities for obtaining the best crystal form.
About this Structure
2H1M is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Synthesis, oxidation behavior, crystallization and structure of 2'-methylseleno guanosine containing RNAs., Moroder H, Kreutz C, Lang K, Serganov A, Micura R, J Am Chem Soc. 2006 Aug 2;128(30):9909-18. PMID:16866550
Page seeded by OCA on Thu Feb 21 17:37:16 2008
