2hah
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | We have obtained the 1.7 A crystal structure of FIV protease (PR) in which | + | We have obtained the 1.7 A crystal structure of FIV protease (PR) in which 12 critical residues around the active site have been substituted with the structurally equivalent residues of HIV PR (12X FIV PR). The chimeric PR was crystallized in complex with the broad-based inhibitor TL-3, which inhibits wild type FIV and HIV PRs, as well as 12X FIV PR and several drug-resistant HIV mutants 1234. Biochemical analyses have demonstrated that TL-3 inhibits these PRs in the order HIV PR > 12X FIV PR > FIV PR, with Ki values of 1.5 nM, 10 nM, and 41 nM, respectively 234. Comparison of the crystal structures of the TL-3 complexes of 12X FIV and wild-typeFIV PR revealed theformation of additinal van der Waals interactions between the enzyme inhibitor in the mutant PR. The 12X FIV PR retained the hydrogen bonding interactions between residues in the flap regions and active site involving the enzyme and the TL-3 inhibitor in comparison to both FIV PR and HIV PR. However, the flap regions of the 12X FIV PR more closely resemble those of HIV PR, having gained several stabilizing intra-flap interactions not present in wild type FIV PR. These findings offer a structural explanation for the observed inhibitor/substrate binding properties of the chimeric PR. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: HIV-1 retropepsin]] | [[Category: HIV-1 retropepsin]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Elder, J | + | [[Category: Elder, J H.]] |
[[Category: Heaslet, H.]] | [[Category: Heaslet, H.]] | ||
| - | [[Category: Lin, Y | + | [[Category: Lin, Y C.]] |
| - | [[Category: Stout, C | + | [[Category: Stout, C D.]] |
[[Category: INT]] | [[Category: INT]] | ||
[[Category: aspartyl]] | [[Category: aspartyl]] | ||
| Line 24: | Line 24: | ||
[[Category: retroviral]] | [[Category: retroviral]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:39:53 2008'' |
Revision as of 15:39, 21 February 2008
|
The structure of FIV 12S protease in complex with TL-3
Overview
We have obtained the 1.7 A crystal structure of FIV protease (PR) in which 12 critical residues around the active site have been substituted with the structurally equivalent residues of HIV PR (12X FIV PR). The chimeric PR was crystallized in complex with the broad-based inhibitor TL-3, which inhibits wild type FIV and HIV PRs, as well as 12X FIV PR and several drug-resistant HIV mutants 1234. Biochemical analyses have demonstrated that TL-3 inhibits these PRs in the order HIV PR > 12X FIV PR > FIV PR, with Ki values of 1.5 nM, 10 nM, and 41 nM, respectively 234. Comparison of the crystal structures of the TL-3 complexes of 12X FIV and wild-typeFIV PR revealed theformation of additinal van der Waals interactions between the enzyme inhibitor in the mutant PR. The 12X FIV PR retained the hydrogen bonding interactions between residues in the flap regions and active site involving the enzyme and the TL-3 inhibitor in comparison to both FIV PR and HIV PR. However, the flap regions of the 12X FIV PR more closely resemble those of HIV PR, having gained several stabilizing intra-flap interactions not present in wild type FIV PR. These findings offer a structural explanation for the observed inhibitor/substrate binding properties of the chimeric PR.
About this Structure
2HAH is a Single protein structure of sequence from Feline immunodeficiency virus with as ligand. Active as HIV-1 retropepsin, with EC number 3.4.23.16 Full crystallographic information is available from OCA.
Reference
Crystal structure of an FIV/HIV chimeric protease complexed with the broad-based inhibitor, TL-3., Heaslet H, Lin YC, Tam K, Torbett BE, Elder JH, Stout CD, Retrovirology. 2007 Jan 9;4:1. PMID:17212810
Page seeded by OCA on Thu Feb 21 17:39:53 2008
