2hb5
From Proteopedia
(New page: 200px<br /><applet load="2hb5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hb5, resolution 1.59Å" /> '''Crystal Structure of...) |
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| - | [[Image:2hb5.gif|left|200px]]<br /><applet load="2hb5" size=" | + | [[Image:2hb5.gif|left|200px]]<br /><applet load="2hb5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hb5, resolution 1.59Å" /> | caption="2hb5, resolution 1.59Å" /> | ||
'''Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain'''<br /> | '''Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A crystallographic study of the Moloney murine leukemia virus (Mo-MLV) | + | A crystallographic study of the Moloney murine leukemia virus (Mo-MLV) RNase H domain was performed to provide information about its structure and mechanism of action. These efforts resulted in the crystallization of a mutant Mo-MLV RNase H lacking the putative helix C (DeltaC). The 1.6-Angstroms resolution structure resembles the known structures of the human immunodeficiency virus type 1 (HIV-1) and Escherichia coli RNase H. The structure revealed the coordination of a magnesium ion within the catalytic core comprised of the highly conserved acidic residues D524, E562, and D583. Surface charge mapping of the Mo-MLV structure revealed a high density of basic charges on one side of the enzyme. Using a model of the Mo-MLV structure superimposed upon a structure of HIV-1 reverse transcriptase bound to an RNA/DNA hybrid substrate, Mo-MLV RNase H secondary structures and individual amino acids were examined for their potential roles in binding substrate. Identified regions included Mo-MLV RNase H beta1-beta2, alphaA, and alphaB and residues from alphaB to alphaD and its following loop. Most of the identified substrate-binding residues corresponded with residues directly binding nucleotides in an RNase H from Bacillus halodurans as observed in a cocrystal structure with RNA/DNA. Finally, superimposition of RNases H of Mo-MLV, E. coli, and HIV-1 revealed that a loop of the HIV-1 connection domain resides within the same region of the Mo-MLV and E. coli C-helix. The HIV-1 connection domain may serve to recognize and bind the RNA/DNA substrate major groove. |
==About this Structure== | ==About this Structure== | ||
| - | 2HB5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus] with MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2HB5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HB5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bingman, C.]] | [[Category: Bingman, C.]] | ||
| - | [[Category: Goff, S | + | [[Category: Goff, S P.]] |
| - | [[Category: Gregorio, G | + | [[Category: Gregorio, G G.]] |
| - | [[Category: Hendrickson, W | + | [[Category: Hendrickson, W A.]] |
[[Category: Lim, D.]] | [[Category: Lim, D.]] | ||
[[Category: Martinez-Hackert, E.]] | [[Category: Martinez-Hackert, E.]] | ||
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[[Category: rnase h]] | [[Category: rnase h]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:03 2008'' |
Revision as of 15:40, 21 February 2008
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Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain
Overview
A crystallographic study of the Moloney murine leukemia virus (Mo-MLV) RNase H domain was performed to provide information about its structure and mechanism of action. These efforts resulted in the crystallization of a mutant Mo-MLV RNase H lacking the putative helix C (DeltaC). The 1.6-Angstroms resolution structure resembles the known structures of the human immunodeficiency virus type 1 (HIV-1) and Escherichia coli RNase H. The structure revealed the coordination of a magnesium ion within the catalytic core comprised of the highly conserved acidic residues D524, E562, and D583. Surface charge mapping of the Mo-MLV structure revealed a high density of basic charges on one side of the enzyme. Using a model of the Mo-MLV structure superimposed upon a structure of HIV-1 reverse transcriptase bound to an RNA/DNA hybrid substrate, Mo-MLV RNase H secondary structures and individual amino acids were examined for their potential roles in binding substrate. Identified regions included Mo-MLV RNase H beta1-beta2, alphaA, and alphaB and residues from alphaB to alphaD and its following loop. Most of the identified substrate-binding residues corresponded with residues directly binding nucleotides in an RNase H from Bacillus halodurans as observed in a cocrystal structure with RNA/DNA. Finally, superimposition of RNases H of Mo-MLV, E. coli, and HIV-1 revealed that a loop of the HIV-1 connection domain resides within the same region of the Mo-MLV and E. coli C-helix. The HIV-1 connection domain may serve to recognize and bind the RNA/DNA substrate major groove.
About this Structure
2HB5 is a Single protein structure of sequence from Moloney murine leukemia virus with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the moloney murine leukemia virus RNase H domain., Lim D, Gregorio GG, Bingman C, Martinez-Hackert E, Hendrickson WA, Goff SP, J Virol. 2006 Sep;80(17):8379-89. PMID:16912289
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