1utd
From Proteopedia
| Line 26: | Line 26: | ||
[[Category: trp rna transcription attenuation]] | [[Category: trp rna transcription attenuation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:10:49 2007'' |
Revision as of 14:06, 30 October 2007
|
THE STRUCTURE OF THE TRP RAN-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 63-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUUU REPEATS
Overview
The trp RNA-binding attenuation protein (TRAP) regulates expression of the, tryptophan biosynthetic genes in several Bacilli by binding to the leader, region of the nascent trp mRNA, inhibiting continued transcription. The 11, subunit TRAP molecule is active in complex with tryptophan, and binds, consequently an RNA target segment consisting of 11 (G/U)AG triplets, each, separated by two or three non-conserved "spacer" nucleotides. Here, we, report the first crystal structures of TRAP in a complex with RNA, containing UAG triplets separated by two nucleotides and in a complex with, RNA containing GAG triplets separated by three nucleotides. Comparison, with known structures of TRAP-RNA complexes shows that both substitution, of G-1 with U-1 in the triplet and addition of an extra spacer ... [(full description)]
About this Structure
1UTD is a [Single protein] structure of sequence from [Geobacillus stearothermophilus] with TRP as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The interaction of RNA with TRAP: the role of triplet repeats and separating spacer nucleotides., Hopcroft NH, Manfredo A, Wendt AL, Brzozowski AM, Gollnick P, Antson AA, J Mol Biol. 2004 Apr 16;338(1):43-53. PMID:15050822
Page seeded by OCA on Tue Oct 30 16:10:49 2007
