2hcy
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C | + | The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C as hexagonal plates or short columns in the presence of NAD+ and 2,2,2-trifluoroethanol, in sodium N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data to 3.2 A resolution show that the crystals are hexagonal in space group P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one subunit of the tetrameric enzyme per asymmetric unit, giving a packing density of 2.9 A3/Da. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Plapp, B | + | [[Category: Plapp, B V.]] |
[[Category: Ramaswamy, S.]] | [[Category: Ramaswamy, S.]] | ||
| - | [[Category: Savarimuthu, B | + | [[Category: Savarimuthu, B R.]] |
[[Category: 8ID]] | [[Category: 8ID]] | ||
[[Category: ETF]] | [[Category: ETF]] | ||
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[[Category: zinc coordination]] | [[Category: zinc coordination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:34 2008'' |
Revision as of 15:40, 21 February 2008
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Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme
Overview
The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C as hexagonal plates or short columns in the presence of NAD+ and 2,2,2-trifluoroethanol, in sodium N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data to 3.2 A resolution show that the crystals are hexagonal in space group P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one subunit of the tetrameric enzyme per asymmetric unit, giving a packing density of 2.9 A3/Da.
About this Structure
2HCY is a Single protein structure of sequence from Saccharomyces cerevisiae with , and as ligands. Active as Alcohol dehydrogenase, with EC number 1.1.1.1 Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I., Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV, J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:8289298
Page seeded by OCA on Thu Feb 21 17:40:34 2008
