2hdc

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(New page: 200px<br /><applet load="2hdc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hdc" /> '''STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DN...)
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'''STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX'''<br />
'''STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX'''<br />
==Overview==
==Overview==
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Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR, techniques, we have obtained the solution structure and backbone dynamics, of Genesis complexed with a 17 base-pair DNA. Our results indicate that, both the local folding and dynamic properties of Genesis are perturbed, when it binds to the DNA site. Our data show that a conserved flexible, amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex, and a short helix is induced by Genesis-DNA interactions. Our data, indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not, required in forming the stable Genesis-DNA complex.
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Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.
==About this Structure==
==About this Structure==
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2HDC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HDC OCA].
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2HDC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HDC OCA].
==Reference==
==Reference==
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[[Category: winged helix protein]]
[[Category: winged helix protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:41:12 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:40 2008''

Revision as of 15:40, 21 February 2008

Image:2hdc.gif

2hdc

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STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX

Overview

Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.

About this Structure

2HDC is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex., Jin C, Marsden I, Chen X, Liao X, J Mol Biol. 1999 Jun 18;289(4):683-90. PMID:10369754

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