2hev
From Proteopedia
(New page: 200px<br /> <applet load="2hev" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hev, resolution 2.41Å" /> '''Crystal structure o...) |
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| - | [[Image:2hev.gif|left|200px]]<br /> | + | [[Image:2hev.gif|left|200px]]<br /><applet load="2hev" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2hev" size=" | + | |
caption="2hev, resolution 2.41Å" /> | caption="2hev, resolution 2.41Å" /> | ||
'''Crystal structure of the complex between OX40L and OX40'''<br /> | '''Crystal structure of the complex between OX40L and OX40'''<br /> | ||
==Overview== | ==Overview== | ||
| - | OX40 is a T cell costimulator activated by OX40L. Blockade of the | + | OX40 is a T cell costimulator activated by OX40L. Blockade of the OX40L-OX40 interaction has ameliorative effects in animal models of T cell pathologies. In order to better understand the interaction between OX40 and OX40L, we have determined the crystal structure of murine OX40L and of the human OX40-OX40L complex at 1.45 and 2.4 A, respectively. These structures show that OX40L is an unusually small member of the tumor necrosis factor superfamily (TNFSF). The arrangement of the OX40L protomers forming the functional trimer is atypical and differs from that of other members by a 15 degrees rotation of each protomer with respect to the trimer axis, resulting in an open assembly. Site-directed changes of the interfacial residues of OX40L suggest this interface lacks a single "hot spot" and that instead, binding energy is dispersed over at least two distinct areas. These structures demonstrate the structural plasticity of TNFSF members and their interactions with receptors. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HEV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2HEV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HEV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Compaan, D | + | [[Category: Compaan, D M.]] |
| - | [[Category: Hymowitz, S | + | [[Category: Hymowitz, S G.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: cytokine]] | [[Category: cytokine]] | ||
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[[Category: tnfsf]] | [[Category: tnfsf]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:41:06 2008'' |
Revision as of 15:41, 21 February 2008
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Crystal structure of the complex between OX40L and OX40
Contents |
Overview
OX40 is a T cell costimulator activated by OX40L. Blockade of the OX40L-OX40 interaction has ameliorative effects in animal models of T cell pathologies. In order to better understand the interaction between OX40 and OX40L, we have determined the crystal structure of murine OX40L and of the human OX40-OX40L complex at 1.45 and 2.4 A, respectively. These structures show that OX40L is an unusually small member of the tumor necrosis factor superfamily (TNFSF). The arrangement of the OX40L protomers forming the functional trimer is atypical and differs from that of other members by a 15 degrees rotation of each protomer with respect to the trimer axis, resulting in an open assembly. Site-directed changes of the interfacial residues of OX40L suggest this interface lacks a single "hot spot" and that instead, binding energy is dispersed over at least two distinct areas. These structures demonstrate the structural plasticity of TNFSF members and their interactions with receptors.
Disease
Known disease associated with this structure: Myocardial infarction, susceptibility to OMIM:[603594]
About this Structure
2HEV is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of the costimulatory OX40-OX40L complex., Compaan DM, Hymowitz SG, Structure. 2006 Aug;14(8):1321-30. PMID:16905106
Page seeded by OCA on Thu Feb 21 17:41:06 2008
