2hki
From Proteopedia
(New page: 200px<br /><applet load="2hki" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hki, resolution 3.00Å" /> '''Crystal structure of...) |
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| - | [[Image:2hki.gif|left|200px]]<br /><applet load="2hki" size=" | + | [[Image:2hki.gif|left|200px]]<br /><applet load="2hki" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hki, resolution 3.00Å" /> | caption="2hki, resolution 3.00Å" /> | ||
'''Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform'''<br /> | '''Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate | + | Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 A and the other of which belonged to a new space group I4(1)22, with unit-cell parameters a = b = 120.9, c = 154.5 A. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 A. Differences at the NAD(+)/NADP(+)-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 2HKI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+))_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.13 1.2.1.13] Full crystallographic information is available from [http:// | + | 2HKI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+))_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.13 1.2.1.13] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HKI OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta | + | Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17077485 17077485] |
[[Category: Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)]] | [[Category: Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:42:49 2008'' |
Revision as of 15:42, 21 February 2008
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Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform
Overview
Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 A and the other of which belonged to a new space group I4(1)22, with unit-cell parameters a = b = 120.9, c = 154.5 A. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 A. Differences at the NAD(+)/NADP(+)-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme.
About this Structure
2HKI is a Single protein structure of sequence from Spinacia oleracea with as ligand. Active as Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating), with EC number 1.2.1.13 Full crystallographic information is available from OCA.
Reference
Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:17077485
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