2hlh
From Proteopedia
(New page: 200px<br /><applet load="2hlh" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hlh, resolution 1.95Å" /> '''Crystal structure of...) |
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caption="2hlh, resolution 1.95Å" /> | caption="2hlh, resolution 1.95Å" /> | ||
'''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium'''<br /> | '''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium'''<br /> | ||
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| + | ==Overview== | ||
| + | The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain. | ||
==About this Structure== | ==About this Structure== | ||
2HLH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_sp. Bradyrhizobium sp.] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glycoprotein_6-alpha-L-fucosyltransferase Glycoprotein 6-alpha-L-fucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.68 2.4.1.68] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HLH OCA]. | 2HLH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_sp. Bradyrhizobium sp.] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glycoprotein_6-alpha-L-fucosyltransferase Glycoprotein 6-alpha-L-fucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.68 2.4.1.68] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HLH OCA]. | ||
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| + | ==Reference== | ||
| + | High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17762900 17762900] | ||
[[Category: Bradyrhizobium sp.]] | [[Category: Bradyrhizobium sp.]] | ||
[[Category: Glycoprotein 6-alpha-L-fucosyltransferase]] | [[Category: Glycoprotein 6-alpha-L-fucosyltransferase]] | ||
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[[Category: nodz]] | [[Category: nodz]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:12 2008'' |
Revision as of 15:43, 21 February 2008
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Crystal structure of fucosyltransferase NodZ from Bradyrhizobium
Overview
The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.
About this Structure
2HLH is a Single protein structure of sequence from Bradyrhizobium sp. with as ligand. Active as Glycoprotein 6-alpha-L-fucosyltransferase, with EC number 2.4.1.68 Full crystallographic information is available from OCA.
Reference
High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:17762900
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