2hnk
From Proteopedia
(New page: 200px<br /><applet load="2hnk" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hnk, resolution 2.300Å" /> '''Crystal structure o...) |
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==Overview== | ==Overview== | ||
| - | The S-adenosylmethionine (SAM)-dependent O-methyltransferase from | + | The S-adenosylmethionine (SAM)-dependent O-methyltransferase from Leptospira interrogans (LiOMT) expressed by gene LA0415 belongs to the Methyltransf_3 family (Pfam PF01596). In this family all of the five bacterial homologues with known function are reported as SAM-dependent O-methylstransferases involved in antibiotic production. The crystal structure of LiOMT in complex with S-adenosylhomocysteine reported here is the first bacterial protein structure in this family. The LiOMT structure shows a conserved SAM-binding region and a probable metal-dependent catalytic site. The molecules of LiOMT generate homodimers by N-terminal swapping, which assists the pre-organization of the substrate-binding site. Based on the sequence and structural analysis, it is implied by the catalytic and substrate-binding site that the substrate of LiOMT is a phenolic derivative, which probably has a large ring-shaped moiety. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Apr 30 | + | Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17561415 17561415] |
[[Category: Leptospira interrogans]] | [[Category: Leptospira interrogans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:46 2008'' |
Revision as of 15:43, 21 February 2008
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Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans
Overview
The S-adenosylmethionine (SAM)-dependent O-methyltransferase from Leptospira interrogans (LiOMT) expressed by gene LA0415 belongs to the Methyltransf_3 family (Pfam PF01596). In this family all of the five bacterial homologues with known function are reported as SAM-dependent O-methylstransferases involved in antibiotic production. The crystal structure of LiOMT in complex with S-adenosylhomocysteine reported here is the first bacterial protein structure in this family. The LiOMT structure shows a conserved SAM-binding region and a probable metal-dependent catalytic site. The molecules of LiOMT generate homodimers by N-terminal swapping, which assists the pre-organization of the substrate-binding site. Based on the sequence and structural analysis, it is implied by the catalytic and substrate-binding site that the substrate of LiOMT is a phenolic derivative, which probably has a large ring-shaped moiety.
About this Structure
2HNK is a Single protein structure of sequence from Leptospira interrogans with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:17561415
Page seeded by OCA on Thu Feb 21 17:43:46 2008
Categories: Leptospira interrogans | Single protein | Gong, W. | Hou, X. | Wei, Z. | PEG | SAH | SO4 | Modified rossman fold | Transferase
