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Thioesterase
From Proteopedia
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| - | '''Thioesterase''' (TE) catalyzes the break of an ester bond to produce acid and alcohol at a thiol group. TEs are substrate-specific. Palmitoyl protein TE removes fatty acids like palmitate from modified cysteine residues during lysosomal degradation. 4-hydroxybenzoyl-CoA TE converts 4-hydroxybenzoyl-CoA to 4-hydroxybenzoate and CoA. Acyl-CoA TE hydrolyzes acyl-CoA to the fatty acid and CoA and is involved in lipid metabolism. Fluoroacetyl-CoA TE from ''Streptomyces cattleya'' hydrolyzes fluoroacetyl-CoA thus preventing it from being metabolized to the lethal 4-hydroxy-trans-aconitate. Ubiquitin TE removes conjugated ubiquitin from proteins thus regulating protein level by preventing their degradation. | + | '''Thioesterase''' (TE) catalyzes the break of an ester bond to produce acid and alcohol at a thiol group. TEs are substrate-specific. '''Palmitoyl protein TE''' removes fatty acids like palmitate from modified cysteine residues during lysosomal degradation. '''4-hydroxybenzoyl-CoA TE''' converts 4-hydroxybenzoyl-CoA to 4-hydroxybenzoate and CoA. '''Acyl-CoA TE''' hydrolyzes acyl-CoA to the fatty acid and CoA and is involved in lipid metabolism. '''Fluoroacetyl-CoA TE''' from ''Streptomyces cattleya'' hydrolyzes fluoroacetyl-CoA thus preventing it from being metabolized to the lethal 4-hydroxy-trans-aconitate. '''Ubiquitin TE''' (USP) removes conjugated ubiquitin from proteins thus regulating protein level by preventing their degradation. |
==3D structures of thioesterase== | ==3D structures of thioesterase== | ||
| + | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
'''Maristoyl-ACP-specific TE''' | '''Maristoyl-ACP-specific TE''' | ||
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'''Ubiquitin TE''' | '''Ubiquitin TE''' | ||
| - | [[ | + | ''USP 2'' |
| - | [[ | + | |
| - | [[ | + | [[2zfy]] – hUSP – human <BR /> |
| + | [[2hd5]], [[3nhe]] – hUSP + Ub <BR /> | ||
| + | [[1tff]] – hUSP (mutant) <BR /> | ||
| + | [[2ibi]] – hUSP (mutant) + Ub <BR /> | ||
| + | |||
| + | ''USP 3'' | ||
| + | |||
| + | [[2qiy]] – USP + UBP-associated protein – yeast <BR /> | ||
| + | |||
| + | ''USP 5'' | ||
| + | |||
| + | [[3ihp]] – hUSP + Ub <BR /> | ||
| + | |||
| + | ''USP 7'' | ||
| + | |||
| + | [[2ylm]] – hUSP <BR /> | ||
| + | [[2kvr]] – hUSP UBL domain - NMR <BR /> | ||
| + | [[3mqr]], [[3mqs]] – hUSP + peptide <BR /> | ||
| + | [[2xxn]] – hUSP + VIRF-4 <BR /> | ||
| + | [[4jjq]] – hUSP (mutant) + E2<BR /> | ||
| + | |||
| + | ''USP 8'' | ||
| + | |||
| + | [[2gwf]] – hUSP rhodanese domain + ring finger protein <BR /> | ||
| + | [[3mhh]], [[3m99]], [[4fip]], [[4fjc]], [[4fk5]] – hUSP + SUS1 + SAGA-associated factors 11, 73 <BR /> | ||
| + | [[3mhs]] – hUSP + SUS1 + SAGA-associated factors 11, 73 + Ub <BR /> | ||
| + | [[3n3k]] – hUSP + Ub inhibitor <BR /> | ||
| + | |||
| + | ''USP 15'' | ||
| + | |||
| + | [[3lmn]] – hUSP DUSP domain <BR /> | ||
| + | |||
| + | ''USP 16'' | ||
| + | |||
| + | [[2i50]] – hUSP zinc finger domain - NMR <BR /> | ||
| + | |||
| + | ''USP 21'' | ||
| + | |||
| + | [[3i3t]] – hUSP + Ub <BR /> | ||
| + | [[3mtn]] – hUSP + Ub inhibitor <BR /> | ||
| + | |||
| + | ''USP 28'' | ||
| + | |||
| + | [[2lva]] – hUSP N terminal - NMR <BR /> | ||
| + | |||
| + | ''USP 33'' | ||
| + | |||
| + | [[2uzg]] – hUSP zinc finger domain - NMR <BR /> | ||
| + | |||
| + | ''USP 37'' | ||
| + | |||
| + | [[3ihr]] – hUSP (mutant) <BR /> | ||
| + | [[3a7s]] – hUSP catalytic domain (mutant) <BR /> | ||
| + | |||
| + | ''USP Cyld'' | ||
| + | |||
| + | [[2vhf]] – hUSP <BR /> | ||
| + | |||
| + | ''USP L1'' | ||
| + | |||
| + | [[3irt]] – hUSP (mutant) <BR /> | ||
| + | [[2len]] – hUSP (mutant) - NMR<BR /> | ||
| + | [[3ifw]], [[3kvf]], [[3kw5]] – hUSP (mutant) + Ub<BR /> | ||
| + | [[4dm9]] – hUSP + peptide inhibitor<BR /> | ||
| + | |||
| + | ''USP L5'' | ||
| + | |||
| + | [[3ris]] – hUSP catalytic domain <BR /> | ||
| + | [[3rii]] – hUSP catalytic domain (mutant) <BR /> | ||
| + | [[3tb3]] – hUSP UCH domain (mutant) <BR /> | ||
| + | |||
| + | ''USP ZranB1'' | ||
| + | |||
| + | [[3zrh]] – hUSP <BR /> | ||
| + | |||
| + | ''USP OTUB1'' | ||
| + | |||
| + | [[3von]] – hUSP + E2 <BR /> | ||
| + | [[4ddg]], [[4ddi]] – hUSP + Ub<BR /> | ||
| + | [[4ddi]] – hUSP (mutant) + Ub<BR /> | ||
| + | [[4dhz]] – hUSP + E2 + Ub<BR /> | ||
| + | [[4dhj]] – nUSP + E2 + Ub - nematode<BR /> | ||
| + | [[4dhi]] – nUSP + E2 <BR /> | ||
| + | |||
| + | [[3znh]] – USP + Ub – Crimean-Congo hemorrhagic fever virus<BR /> | ||
'''RedJ TE''' | '''RedJ TE''' | ||
Revision as of 10:37, 25 June 2013
Thioesterase (TE) catalyzes the break of an ester bond to produce acid and alcohol at a thiol group. TEs are substrate-specific. Palmitoyl protein TE removes fatty acids like palmitate from modified cysteine residues during lysosomal degradation. 4-hydroxybenzoyl-CoA TE converts 4-hydroxybenzoyl-CoA to 4-hydroxybenzoate and CoA. Acyl-CoA TE hydrolyzes acyl-CoA to the fatty acid and CoA and is involved in lipid metabolism. Fluoroacetyl-CoA TE from Streptomyces cattleya hydrolyzes fluoroacetyl-CoA thus preventing it from being metabolized to the lethal 4-hydroxy-trans-aconitate. Ubiquitin TE (USP) removes conjugated ubiquitin from proteins thus regulating protein level by preventing their degradation.
3D structures of thioesterase
Updated on 25-June-2013
Maristoyl-ACP-specific TE
1tht – TE – Vibrio harveyi
4-hydroxybenzoyl-CoA TE
1bvq – PsTE – Pseudomonas
1lo7 – PsTE + 4-hydroxyphenyl CoA
1lo9 - PsTE (mutant) + 4-hydroxybenzoyl CoA
1lo8 - PsTE + 4-hydroxybenzyl CoA
1q4s - ArTE + 4-hydroxybenzoate – 'Arthrobacter'
1q4t - ArTE + 4-hydroxyphenyl CoA
1q4u - ArTE + 4-hydroxybenzyl CoA
3r32, 3r35, 3r37, 3r38, 3r3c, 3r3d, 3r3f - ArTE (mutant) + 4-hydroxyphenacyl CoA
3r3a - ArTE (mutant) + 4-hydroxybenzoate
3r34 - ArTE (mutant) + CoA
Palmitoyl protein TE
1eh5 – bTE1 + Palmitate – bovine
1ei9 – bTE1
1exw – bTE1 + hexadecylsulfonyl fluoride
1pja – hTEII – human
3gro - hTEI
Acyl-CoA TE
1c8u – EcTEII – Escherichia coli
1ivn – EcTEI
1jrl – EcTEI (mutant)
1j00 – EcTEI + diethyl phosphono derivative
1v2g, 1u8u - EcTEI + octanoic acid
2v1o – mTE7 hotdog domain – mouse
2q2b – mTE7 C terminal
3hlk – hTE2
3k2i – hTE4
2qq2 - hTE7 C terminal
3fo5 – hTE11 START domain
3b7k – hTE12
3rd7 – TE – Mycobacterium avium
3u0a – TEII – Mycobacterium marinum
1tbu – TE N terminal (peroximal) – yeast
Acyl protein TE
1fj2 – EcTEI
Acyl-ACP TE
2ess – TE – Bacterioides thetaiotaomicron
Fluoroacetyl-CoA TE
3kuv, 3kuw, 3kvi – ScTE (mutant) + acetate derivative – Streptomyces cattleya
3kv7, 3kv8, 3p2r, 3p2s – ScTE + acetate derivative
3kvu – ScTE + acetyl-CoA
3kvz – ScTE + FAcOPan
3kx7, 3fx8, 3p2q – ScTE
3p3f – ScTE (mutant)
3p3i - ScTE (mutant) + acetate derivative + CoA
Ubiquitin TE
USP 2
2zfy – hUSP – human
2hd5, 3nhe – hUSP + Ub
1tff – hUSP (mutant)
2ibi – hUSP (mutant) + Ub
USP 3
2qiy – USP + UBP-associated protein – yeast
USP 5
3ihp – hUSP + Ub
USP 7
2ylm – hUSP
2kvr – hUSP UBL domain - NMR
3mqr, 3mqs – hUSP + peptide
2xxn – hUSP + VIRF-4
4jjq – hUSP (mutant) + E2
USP 8
2gwf – hUSP rhodanese domain + ring finger protein
3mhh, 3m99, 4fip, 4fjc, 4fk5 – hUSP + SUS1 + SAGA-associated factors 11, 73
3mhs – hUSP + SUS1 + SAGA-associated factors 11, 73 + Ub
3n3k – hUSP + Ub inhibitor
USP 15
3lmn – hUSP DUSP domain
USP 16
2i50 – hUSP zinc finger domain - NMR
USP 21
3i3t – hUSP + Ub
3mtn – hUSP + Ub inhibitor
USP 28
2lva – hUSP N terminal - NMR
USP 33
2uzg – hUSP zinc finger domain - NMR
USP 37
3ihr – hUSP (mutant)
3a7s – hUSP catalytic domain (mutant)
USP Cyld
2vhf – hUSP
USP L1
3irt – hUSP (mutant)
2len – hUSP (mutant) - NMR
3ifw, 3kvf, 3kw5 – hUSP (mutant) + Ub
4dm9 – hUSP + peptide inhibitor
USP L5
3ris – hUSP catalytic domain
3rii – hUSP catalytic domain (mutant)
3tb3 – hUSP UCH domain (mutant)
USP ZranB1
3zrh – hUSP
USP OTUB1
3von – hUSP + E2
4ddg, 4ddi – hUSP + Ub
4ddi – hUSP (mutant) + Ub
4dhz – hUSP + E2 + Ub
4dhj – nUSP + E2 + Ub - nematode
4dhi – nUSP + E2
3znh – USP + Ub – Crimean-Congo hemorrhagic fever virus
RedJ TE
3qmv, 3qmw – TE – Streptomyces coelicolor
2av9 – TE – Pseudomonas aeruginosa
