3eh8
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:3eh8.png|left|200px]] | ||
| - | |||
{{STRUCTURE_3eh8| PDB=3eh8 | SCENE= }} | {{STRUCTURE_3eh8| PDB=3eh8 | SCENE= }} | ||
| - | |||
===Crystal structure of Y2 I-AniI variant (F13Y/S111Y)/DNA complex with calcium=== | ===Crystal structure of Y2 I-AniI variant (F13Y/S111Y)/DNA complex with calcium=== | ||
| - | |||
{{ABSTRACT_PUBMED_19103658}} | {{ABSTRACT_PUBMED_19103658}} | ||
| + | |||
| + | ==Function== | ||
| + | [[http://www.uniprot.org/uniprot/ANI1_EMEND ANI1_EMEND]] Mitochondrial DNA endonuclease and mRNA maturase involved in intron homing and required for splicing of the cytochrome b (cobA) gene intron, containing its own coding sequence. The protein stimulates the intrinsic ribozyme activity of the intron through binding to and stabilizing specific secondary and tertiary structure elements in the RNA. As an endonuclease it introduces a specific double-strand break at the junction of the two exons the cobA gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with limited specificity and cleaves the sequence 5'-GAGGAGGTTTCTCTGTA-3'. The proteins RNA and DNA recognition and binding surfaces are independent.<ref>PMID:9256423</ref> <ref>PMID:10512698</ref> <ref>PMID:12758073</ref> | ||
==About this Structure== | ==About this Structure== | ||
[[3eh8]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EH8 OCA]. | [[3eh8]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EH8 OCA]. | ||
| - | |||
| - | ==See Also== | ||
| - | *[[Endonuclease|Endonuclease]] | ||
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:019103658</ref><references group="xtra"/> | + | <ref group="xtra">PMID:019103658</ref><references group="xtra"/><references/> |
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
Revision as of 07:14, 30 June 2013
Contents |
Crystal structure of Y2 I-AniI variant (F13Y/S111Y)/DNA complex with calcium
Template:ABSTRACT PUBMED 19103658
Function
[ANI1_EMEND] Mitochondrial DNA endonuclease and mRNA maturase involved in intron homing and required for splicing of the cytochrome b (cobA) gene intron, containing its own coding sequence. The protein stimulates the intrinsic ribozyme activity of the intron through binding to and stabilizing specific secondary and tertiary structure elements in the RNA. As an endonuclease it introduces a specific double-strand break at the junction of the two exons the cobA gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with limited specificity and cleaves the sequence 5'-GAGGAGGTTTCTCTGTA-3'. The proteins RNA and DNA recognition and binding surfaces are independent.[1] [2] [3]
About this Structure
3eh8 is a 9 chain structure with sequence from Emericella nidulans. Full crystallographic information is available from OCA.
Reference
- Takeuchi R, Certo M, Caprara MG, Scharenberg AM, Stoddard BL. Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation. Nucleic Acids Res. 2008 Dec 22. PMID:19103658 doi:gkn1007
- ↑ Ho Y, Kim SJ, Waring RB. A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease. Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8994-9. PMID:9256423
- ↑ Ho Y, Waring RB. The maturase encoded by a group I intron from Aspergillus nidulans stabilizes RNA tertiary structure and promotes rapid splicing. J Mol Biol. 1999 Oct 8;292(5):987-1001. PMID:10512698 doi:10.1006/jmbi.1999.3070
- ↑ Chatterjee P, Brady KL, Solem A, Ho Y, Caprara MG. Functionally distinct nucleic acid binding sites for a group I intron encoded RNA maturase/DNA homing endonuclease. J Mol Biol. 2003 May 30;329(2):239-51. PMID:12758073
