2htj
From Proteopedia
(New page: 200px<br /><applet load="2htj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2htj" /> '''NMR structure of E.coli PapI'''<br /> ==Ove...) |
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| - | [[Image:2htj.jpg|left|200px]]<br /><applet load="2htj" size=" | + | [[Image:2htj.jpg|left|200px]]<br /><applet load="2htj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2htj" /> | caption="2htj" /> | ||
'''NMR structure of E.coli PapI'''<br /> | '''NMR structure of E.coli PapI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli | + | Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli to bind to epithelial cells and play an important role in urinary tract infection. Expression of pap is controlled by a phase-variation mechanism, based on the two distinct heritable states that are the result of adenine N6-methylation in either of the two GATC sequences in its regulatory region. The methylation status of these two sequences is sensed by the action of two proteins, Lrp and PapI, and they play a central role in determining pap gene expression in both phase-ON and phase-OFF cells. We used modern NMR techniques to determine the solution structure and backbone dynamics of PapI. We found its overall fold resembles closely that of the winged helix-turn-helix family of DNA-binding proteins. We determined that PapI possesses its own DNA-binding activity, albeit non-sequence-specific, independent of Lrp. PapI appears to bind to DNA with a K(d) in the 10 microM range. Possible mechanisms by which PapI might participate in the regulation of the pap operon are discussed in light of these new findings. |
==About this Structure== | ==About this Structure== | ||
| - | 2HTJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2HTJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HTJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dahlquist, F | + | [[Category: Dahlquist, F W.]] |
[[Category: Kawamura, T.]] | [[Category: Kawamura, T.]] | ||
| - | [[Category: Le, L | + | [[Category: Le, L U.K.]] |
[[Category: Zhou, H.]] | [[Category: Zhou, H.]] | ||
[[Category: pap pili]] | [[Category: pap pili]] | ||
[[Category: winged helix-turn-helix]] | [[Category: winged helix-turn-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:45:35 2008'' |
Revision as of 15:45, 21 February 2008
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NMR structure of E.coli PapI
Overview
Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli to bind to epithelial cells and play an important role in urinary tract infection. Expression of pap is controlled by a phase-variation mechanism, based on the two distinct heritable states that are the result of adenine N6-methylation in either of the two GATC sequences in its regulatory region. The methylation status of these two sequences is sensed by the action of two proteins, Lrp and PapI, and they play a central role in determining pap gene expression in both phase-ON and phase-OFF cells. We used modern NMR techniques to determine the solution structure and backbone dynamics of PapI. We found its overall fold resembles closely that of the winged helix-turn-helix family of DNA-binding proteins. We determined that PapI possesses its own DNA-binding activity, albeit non-sequence-specific, independent of Lrp. PapI appears to bind to DNA with a K(d) in the 10 microM range. Possible mechanisms by which PapI might participate in the regulation of the pap operon are discussed in light of these new findings.
About this Structure
2HTJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Solution structure of Escherichia coli PapI, a key regulator of the pap pili phase variation., Kawamura T, Le LU, Zhou H, Dahlquist FW, J Mol Biol. 2007 Jan 26;365(4):1130-42. Epub 2006 Oct 25. PMID:17109885
Page seeded by OCA on Thu Feb 21 17:45:35 2008
