2hvd
From Proteopedia
(New page: 200px<br /> <applet load="2hvd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hvd, resolution 2.15Å" /> '''Human nucleoside di...) |
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| - | [[Image:2hvd.gif|left|200px]]<br /> | + | [[Image:2hvd.gif|left|200px]]<br /><applet load="2hvd" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2hvd" size=" | + | |
caption="2hvd, resolution 2.15Å" /> | caption="2hvd, resolution 2.15Å" /> | ||
'''Human nucleoside diphosphate kinase A complexed with ADP'''<br /> | '''Human nucleoside diphosphate kinase A complexed with ADP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nm23 was the first metastasis suppressor gene identified. This gene | + | Nm23 was the first metastasis suppressor gene identified. This gene encodes a NDP kinase that also exhibits other properties like histidine protein kinase and interactions with proteins and DNA. The S120G mutant of NDPK-A has been identified in aggressive neuroblastomas and has been found to reduce the metastasis suppressor effect of Nm23. In order to understand the differences between the wild type and the S120G mutant, we have determined the structure of both mutant and wild type NDPK-A in complex with ADP. Our results reveal that there are no significant changes between the two enzyme versions even in the surroundings of the catalytic histidine that is required for NDP kinase activity. This suggests that the S120G mutation may affect an other protein property than NDP kinase activity. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HVD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Full crystallographic information is available from [http:// | + | 2HVD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HVD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dautant, A.]] | [[Category: Dautant, A.]] | ||
[[Category: Georgescauld, F.]] | [[Category: Georgescauld, F.]] | ||
| - | [[Category: Giraud, M | + | [[Category: Giraud, M F.]] |
[[Category: Lascu, I.]] | [[Category: Lascu, I.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: complex adp]] | [[Category: complex adp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:46:08 2008'' |
Revision as of 15:46, 21 February 2008
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Human nucleoside diphosphate kinase A complexed with ADP
Contents |
Overview
Nm23 was the first metastasis suppressor gene identified. This gene encodes a NDP kinase that also exhibits other properties like histidine protein kinase and interactions with proteins and DNA. The S120G mutant of NDPK-A has been identified in aggressive neuroblastomas and has been found to reduce the metastasis suppressor effect of Nm23. In order to understand the differences between the wild type and the S120G mutant, we have determined the structure of both mutant and wild type NDPK-A in complex with ADP. Our results reveal that there are no significant changes between the two enzyme versions even in the surroundings of the catalytic histidine that is required for NDP kinase activity. This suggests that the S120G mutation may affect an other protein property than NDP kinase activity.
Disease
Known disease associated with this structure: Neuroblastoma OMIM:[156490]
About this Structure
2HVD is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Nucleoside-diphosphate kinase, with EC number 2.7.4.6 Full crystallographic information is available from OCA.
Reference
Crystal structures of S120G mutant and wild type of human nucleoside diphosphate kinase A in complex with ADP., Giraud MF, Georgescauld F, Lascu I, Dautant A, J Bioenerg Biomembr. 2006 Aug;38(3-4):261-4. Epub 2006 Sep 1. PMID:16944299
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