2hyi
From Proteopedia
(New page: 200px<br /> <applet load="2hyi" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hyi, resolution 2.3Å" /> '''Structure of the hum...) |
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| - | [[Image:2hyi.gif|left|200px]]<br /> | + | [[Image:2hyi.gif|left|200px]]<br /><applet load="2hyi" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2hyi" size=" | + | |
caption="2hyi, resolution 2.3Å" /> | caption="2hyi, resolution 2.3Å" /> | ||
'''Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA'''<br /> | '''Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In higher eukaryotes, a multiprotein exon junction complex is deposited on | + | In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I. |
==About this Structure== | ==About this Structure== | ||
| - | 2HYI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2HYI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HYI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Andersen, C | + | [[Category: Andersen, C B.F.]] |
| - | [[Category: Andersen, G | + | [[Category: Andersen, G R.]] |
| - | [[Category: Hir, H | + | [[Category: Hir, H Le.]] |
[[Category: ANP]] | [[Category: ANP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: translation]] | [[Category: translation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:47:08 2008'' |
Revision as of 15:47, 21 February 2008
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Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA
Overview
In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.
About this Structure
2HYI is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA., Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H, Andersen GR, Science. 2006 Sep 29;313(5795):1968-72. Epub 2006 Aug 24. PMID:16931718
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