2hza
From Proteopedia
(New page: 200px<br /><applet load="2hza" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hza, resolution 2.10Å" /> '''Nickel-bound full-le...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2hza.gif|left|200px]]<br /><applet load="2hza" size=" | + | [[Image:2hza.gif|left|200px]]<br /><applet load="2hza" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2hza, resolution 2.10Å" /> | caption="2hza, resolution 2.10Å" /> | ||
'''Nickel-bound full-length Escherichia coli NikR'''<br /> | '''Nickel-bound full-length Escherichia coli NikR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Metal ion homeostasis is critical to the survival of all cells. Regulation | + | Metal ion homeostasis is critical to the survival of all cells. Regulation of nickel concentrations in Escherichia coli is mediated by the NikR repressor via nickel-induced transcriptional repression of the nickel ABC-type transporter, NikABCDE. Here, we report two crystal structures of nickel-activated E. coli NikR, the isolated repressor at 2.1 A resolution and in a complex with its operator DNA sequence from the nik promoter at 3.1 A resolution. Along with the previously published structure of apo-NikR, these structures allow us to evaluate functional proposals for how metal ions activate NikR, delineate the drastic conformational changes required for operator recognition, and describe the formation of a second metal-binding site in the presence of DNA. They also provide a rare set of structural views of a ligand-responsive transcription factor in the unbound, ligand-induced, and DNA-bound states, establishing a model system for the study of ligand-mediated effects on transcription factor function. |
==About this Structure== | ==About this Structure== | ||
| - | 2HZA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with 3CM and NI as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2HZA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=3CM:'>3CM</scene> and <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZA OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Drennan, C | + | [[Category: Drennan, C L.]] |
| - | [[Category: Schreiter, E | + | [[Category: Schreiter, E R.]] |
[[Category: 3CM]] | [[Category: 3CM]] | ||
[[Category: NI]] | [[Category: NI]] | ||
| Line 21: | Line 21: | ||
[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:47:24 2008'' |
Revision as of 15:47, 21 February 2008
|
Nickel-bound full-length Escherichia coli NikR
Overview
Metal ion homeostasis is critical to the survival of all cells. Regulation of nickel concentrations in Escherichia coli is mediated by the NikR repressor via nickel-induced transcriptional repression of the nickel ABC-type transporter, NikABCDE. Here, we report two crystal structures of nickel-activated E. coli NikR, the isolated repressor at 2.1 A resolution and in a complex with its operator DNA sequence from the nik promoter at 3.1 A resolution. Along with the previously published structure of apo-NikR, these structures allow us to evaluate functional proposals for how metal ions activate NikR, delineate the drastic conformational changes required for operator recognition, and describe the formation of a second metal-binding site in the presence of DNA. They also provide a rare set of structural views of a ligand-responsive transcription factor in the unbound, ligand-induced, and DNA-bound states, establishing a model system for the study of ligand-mediated effects on transcription factor function.
About this Structure
2HZA is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
Reference
NikR-operator complex structure and the mechanism of repressor activation by metal ions., Schreiter ER, Wang SC, Zamble DB, Drennan CL, Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13676-81. Epub 2006 Aug 31. PMID:16945905
Page seeded by OCA on Thu Feb 21 17:47:24 2008
