2i2y
From Proteopedia
(New page: 200px<br /> <applet load="2i2y" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i2y" /> '''Solution structure of the RRM of SRp20 boun...) |
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'''Solution structure of the RRM of SRp20 bound to the RNA CAUC'''<br /> | '''Solution structure of the RRM of SRp20 bound to the RNA CAUC'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest | + | The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8. |
==About this Structure== | ==About this Structure== | ||
| - | 2I2Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._group_g_and_homo_sapiens Streptococcus sp. group g and homo sapiens]. Full crystallographic information is available from [http:// | + | 2I2Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._group_g_and_homo_sapiens Streptococcus sp. group g and homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptococcus sp. group g and homo sapiens]] | [[Category: Streptococcus sp. group g and homo sapiens]] | ||
| - | [[Category: Allain, F | + | [[Category: Allain, F H.]] |
| - | [[Category: Hargous, Y | + | [[Category: Hargous, Y F.]] |
[[Category: protein-rna complex rrm alpha-beta sandwich beta1-alpha1-beta2-beta3-alpha2-beta4]] | [[Category: protein-rna complex rrm alpha-beta sandwich beta1-alpha1-beta2-beta3-alpha2-beta4]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:48:30 2008'' |
Revision as of 15:48, 21 February 2008
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Solution structure of the RRM of SRp20 bound to the RNA CAUC
Overview
The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8.
About this Structure
2I2Y is a Single protein structure of sequence from Streptococcus sp. group g and homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8., Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH, EMBO J. 2006 Nov 1;25(21):5126-37. Epub 2006 Oct 12. PMID:17036044
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