2i9t
From Proteopedia
(New page: 200px<br /><applet load="2i9t" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i9t, resolution 2.80Å" /> '''Structure of NF-kB p...) |
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| - | [[Image:2i9t.gif|left|200px]]<br /><applet load="2i9t" size=" | + | [[Image:2i9t.gif|left|200px]]<br /><applet load="2i9t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2i9t, resolution 2.80Å" /> | caption="2i9t, resolution 2.80Å" /> | ||
'''Structure of NF-kB p65-p50 heterodimer bound to PRDII element of B-interferon promoter'''<br /> | '''Structure of NF-kB p65-p50 heterodimer bound to PRDII element of B-interferon promoter'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Upon viral infection, NF-kappaB translocates to the nucleus and activates | + | Upon viral infection, NF-kappaB translocates to the nucleus and activates the IFN-beta gene by binding to the PRDII element. Strikingly, NF-kappaB loses its ability to activate the IFN-beta gene when the PRDII element is substituted by closely related sites. We report here the crystal structure of NF-kappaB p50/p65 heterodimer bound to the PRDII element from the IFN-beta promoter. The structure reveals an unexpected alteration in configuration, in which the p50 specificity domain moves by as much as approximately 9 A when compared to NF-kappaB heterodimer bound to the immunoglobulin kappaB site (Ig-kappaB) while maintaining the same base-specific contacts with the DNA. Taken together, the structure offers new insights into the allosteric effects of closely related DNA sites on the configuration of NF-kappaB and its transcriptional selectivity. |
==About this Structure== | ==About this Structure== | ||
| - | 2I9T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 2I9T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Aggarwal, A | + | [[Category: Aggarwal, A K.]] |
| - | [[Category: Escalante, C | + | [[Category: Escalante, C R]] |
[[Category: Shen, L.]] | [[Category: Shen, L.]] | ||
[[Category: Thanos, D.]] | [[Category: Thanos, D.]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:50:24 2008'' |
Revision as of 15:50, 21 February 2008
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Structure of NF-kB p65-p50 heterodimer bound to PRDII element of B-interferon promoter
Overview
Upon viral infection, NF-kappaB translocates to the nucleus and activates the IFN-beta gene by binding to the PRDII element. Strikingly, NF-kappaB loses its ability to activate the IFN-beta gene when the PRDII element is substituted by closely related sites. We report here the crystal structure of NF-kappaB p50/p65 heterodimer bound to the PRDII element from the IFN-beta promoter. The structure reveals an unexpected alteration in configuration, in which the p50 specificity domain moves by as much as approximately 9 A when compared to NF-kappaB heterodimer bound to the immunoglobulin kappaB site (Ig-kappaB) while maintaining the same base-specific contacts with the DNA. Taken together, the structure offers new insights into the allosteric effects of closely related DNA sites on the configuration of NF-kappaB and its transcriptional selectivity.
About this Structure
2I9T is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of NF-kappaB p50/p65 heterodimer bound to the PRDII DNA element from the interferon-beta promoter., Escalante CR, Shen L, Thanos D, Aggarwal AK, Structure. 2002 Mar;10(3):383-91. PMID:12005436
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