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2iho
From Proteopedia
(New page: 200px<br /><applet load="2iho" size="350" color="white" frame="true" align="right" spinBox="true" caption="2iho, resolution 2.41Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | MOA, a lectin from the mushroom Marasmius oreades, is one of the few | + | MOA, a lectin from the mushroom Marasmius oreades, is one of the few reagents that specifically agglutinate blood group B erythrocytes. Further, it is the only lectin known to have exclusive specificity for Galalpha(1,3)Gal-containing sugar epitopes, which are antigens that pose a severe barrier to animal-to-human organ transplantation. We describe here the structure of MOA at 2.4 A resolution, in complex with the linear trisaccharide Galalpha(1,3)Galbeta(1,4)GlcNAc. The structure is dimeric, with two distinct domains per protomer: the N-terminal lectin module adopts a ricinB/beta-trefoil fold and contains three putative carbohydrate-binding sites, while the C-terminal domain serves as a dimerization interface. This latter domain, which has an unknown function, reveals a novel fold with intriguing conservation of an active site cleft. A number of indications suggest that MOA may have an enzymatic function in addition to the sugar-binding properties. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | Crystal structure of the | + | Crystal structure of the Marasmius oreades mushroom lectin in complex with a xenotransplantation epitope., Grahn E, Askarieh G, Holmner A, Tateno H, Winter HC, Goldstein IJ, Krengel U, J Mol Biol. 2007 Jun 8;369(3):710-21. Epub 2007 Mar 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17442345 17442345] |
[[Category: Marasmius oreades]] | [[Category: Marasmius oreades]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Askarieh, G.]] | [[Category: Askarieh, G.]] | ||
| - | [[Category: Goldstein, I | + | [[Category: Goldstein, I J.]] |
[[Category: Grahn, E.]] | [[Category: Grahn, E.]] | ||
[[Category: Holmner, A.]] | [[Category: Holmner, A.]] | ||
[[Category: Krengel, U.]] | [[Category: Krengel, U.]] | ||
[[Category: Tateno, H.]] | [[Category: Tateno, H.]] | ||
| - | [[Category: Winter, H | + | [[Category: Winter, H C.]] |
[[Category: beta-trefoil]] | [[Category: beta-trefoil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:52:47 2008'' |
Revision as of 15:52, 21 February 2008
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Crystal structure of MOA, a lectin from the mushroom Marasmius oreades in complex with the trisaccharide Gal(1,3)Gal(1,4)GlcNAc
Overview
MOA, a lectin from the mushroom Marasmius oreades, is one of the few reagents that specifically agglutinate blood group B erythrocytes. Further, it is the only lectin known to have exclusive specificity for Galalpha(1,3)Gal-containing sugar epitopes, which are antigens that pose a severe barrier to animal-to-human organ transplantation. We describe here the structure of MOA at 2.4 A resolution, in complex with the linear trisaccharide Galalpha(1,3)Galbeta(1,4)GlcNAc. The structure is dimeric, with two distinct domains per protomer: the N-terminal lectin module adopts a ricinB/beta-trefoil fold and contains three putative carbohydrate-binding sites, while the C-terminal domain serves as a dimerization interface. This latter domain, which has an unknown function, reveals a novel fold with intriguing conservation of an active site cleft. A number of indications suggest that MOA may have an enzymatic function in addition to the sugar-binding properties.
About this Structure
2IHO is a Single protein structure of sequence from Marasmius oreades. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Marasmius oreades mushroom lectin in complex with a xenotransplantation epitope., Grahn E, Askarieh G, Holmner A, Tateno H, Winter HC, Goldstein IJ, Krengel U, J Mol Biol. 2007 Jun 8;369(3):710-21. Epub 2007 Mar 15. PMID:17442345
Page seeded by OCA on Thu Feb 21 17:52:47 2008
