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2ipy
From Proteopedia
(New page: 200px<br /> <applet load="2ipy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ipy, resolution 2.80Å" /> '''crystal structure o...) |
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| - | [[Image:2ipy.gif|left|200px]]<br /> | + | [[Image:2ipy.gif|left|200px]]<br /><applet load="2ipy" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2ipy" size=" | + | |
caption="2ipy, resolution 2.80Å" /> | caption="2ipy, resolution 2.80Å" /> | ||
'''crystal structure of iron regulatory protein 1 in complex with ferritin H IRE-RNA'''<br /> | '''crystal structure of iron regulatory protein 1 in complex with ferritin H IRE-RNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Iron regulatory protein 1 (IRP1) binds iron-responsive elements (IREs) in | + | Iron regulatory protein 1 (IRP1) binds iron-responsive elements (IREs) in messenger RNAs (mRNAs), to repress translation or degradation, or binds an iron-sulfur cluster, to become a cytosolic aconitase enzyme. The 2.8 angstrom resolution crystal structure of the IRP1:ferritin H IRE complex shows an open protein conformation compared with that of cytosolic aconitase. The extended, L-shaped IRP1 molecule embraces the IRE stem-loop through interactions at two sites separated by approximately 30 angstroms, each involving about a dozen protein:RNA bonds. Extensive conformational changes related to binding the IRE or an iron-sulfur cluster explain the alternate functions of IRP1 as an mRNA regulator or enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 2IPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. The following page contains interesting information on the relation of 2IPY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb89_1.html Aconitase and Iron Regulatory Protein 1]]. Active as [http://en.wikipedia.org/wiki/Aconitate_hydratase Aconitate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.3 4.2.1.3] Full crystallographic information is available from [http:// | + | 2IPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. The following page contains interesting information on the relation of 2IPY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb89_1.html Aconitase and Iron Regulatory Protein 1]]. Active as [http://en.wikipedia.org/wiki/Aconitate_hydratase Aconitate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.3 4.2.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IPY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dupuy, J.]] | [[Category: Dupuy, J.]] | ||
| - | [[Category: Fontecilla-Camps, J | + | [[Category: Fontecilla-Camps, J C.]] |
| - | [[Category: Selezneva, A | + | [[Category: Selezneva, A I.]] |
| - | [[Category: Theil, E | + | [[Category: Theil, E C.]] |
[[Category: Volbeda, A.]] | [[Category: Volbeda, A.]] | ||
[[Category: Volz, K.]] | [[Category: Volz, K.]] | ||
| - | [[Category: Walden, W | + | [[Category: Walden, W E.]] |
[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:54:57 2008'' |
Revision as of 15:54, 21 February 2008
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crystal structure of iron regulatory protein 1 in complex with ferritin H IRE-RNA
Overview
Iron regulatory protein 1 (IRP1) binds iron-responsive elements (IREs) in messenger RNAs (mRNAs), to repress translation or degradation, or binds an iron-sulfur cluster, to become a cytosolic aconitase enzyme. The 2.8 angstrom resolution crystal structure of the IRP1:ferritin H IRE complex shows an open protein conformation compared with that of cytosolic aconitase. The extended, L-shaped IRP1 molecule embraces the IRE stem-loop through interactions at two sites separated by approximately 30 angstroms, each involving about a dozen protein:RNA bonds. Extensive conformational changes related to binding the IRE or an iron-sulfur cluster explain the alternate functions of IRP1 as an mRNA regulator or enzyme.
About this Structure
2IPY is a Single protein structure of sequence from Oryctolagus cuniculus. The following page contains interesting information on the relation of 2IPY with [Aconitase and Iron Regulatory Protein 1]. Active as Aconitate hydratase, with EC number 4.2.1.3 Full crystallographic information is available from OCA.
Reference
Structure of dual function iron regulatory protein 1 complexed with ferritin IRE-RNA., Walden WE, Selezneva AI, Dupuy J, Volbeda A, Fontecilla-Camps JC, Theil EC, Volz K, Science. 2006 Dec 22;314(5807):1903-8. PMID:17185597
Page seeded by OCA on Thu Feb 21 17:54:57 2008
