2iu1
From Proteopedia
(New page: 200px<br /> <applet load="2iu1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iu1, resolution 1.80Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:2iu1.gif|left|200px]]<br /> | + | [[Image:2iu1.gif|left|200px]]<br /><applet load="2iu1" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2iu1" size=" | + | |
caption="2iu1, resolution 1.80Å" /> | caption="2iu1, resolution 1.80Å" /> | ||
'''CRYSTAL STRUCTURE OF EIF5 C-TERMINAL DOMAIN'''<br /> | '''CRYSTAL STRUCTURE OF EIF5 C-TERMINAL DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The carboxy-terminal domain (CTD) of eukaryotic initiation factor 5 (eIF5) | + | The carboxy-terminal domain (CTD) of eukaryotic initiation factor 5 (eIF5) plays a central role in the formation of the multifactor complex (MFC), an important intermediate for the 43 S pre-initiation complex assembly. The IF5-CTD interacts directly with the translation initiation factors eIF1, eIF2-beta, and eIF3c, thus forming together with eIF2 bound Met-tRNA(i)(Met) the MFC. In this work we present the high resolution crystal structure of eIF5-CTD. This domain of the protein is exclusively composed out of alpha-helices and is homologous to the carboxy-terminal domain of eIF2B-epsilon (eIF2Bepsilon-CTD). The most striking difference in the two structures is an additional carboxy-terminal helix in eIF5. The binding sites of eIF2-beta, eIF3 and eIF1 were mapped onto the structure. eIF2-beta and eIF3 bind to non-overlapping patches of negative and positive electrostatic potential, respectively. |
==About this Structure== | ==About this Structure== | ||
| - | 2IU1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2IU1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: translation inititation]] | [[Category: translation inititation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:55:57 2008'' |
Revision as of 15:55, 21 February 2008
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CRYSTAL STRUCTURE OF EIF5 C-TERMINAL DOMAIN
Overview
The carboxy-terminal domain (CTD) of eukaryotic initiation factor 5 (eIF5) plays a central role in the formation of the multifactor complex (MFC), an important intermediate for the 43 S pre-initiation complex assembly. The IF5-CTD interacts directly with the translation initiation factors eIF1, eIF2-beta, and eIF3c, thus forming together with eIF2 bound Met-tRNA(i)(Met) the MFC. In this work we present the high resolution crystal structure of eIF5-CTD. This domain of the protein is exclusively composed out of alpha-helices and is homologous to the carboxy-terminal domain of eIF2B-epsilon (eIF2Bepsilon-CTD). The most striking difference in the two structures is an additional carboxy-terminal helix in eIF5. The binding sites of eIF2-beta, eIF3 and eIF1 were mapped onto the structure. eIF2-beta and eIF3 bind to non-overlapping patches of negative and positive electrostatic potential, respectively.
About this Structure
2IU1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5., Bieniossek C, Schutz P, Bumann M, Limacher A, Uson I, Baumann U, J Mol Biol. 2006 Jul 7;360(2):457-65. Epub 2006 May 24. PMID:16781736
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