2iw5
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Histone methylation regulates diverse chromatin-templated processes, including transcription. Many transcriptional corepressor complexes | + | Histone methylation regulates diverse chromatin-templated processes, including transcription. Many transcriptional corepressor complexes contain lysine-specific demethylase 1 (LSD1) and CoREST that collaborate to demethylate mono- and dimethylated H3-K4 of nucleosomes. Here, we report the crystal structure of the LSD1-CoREST complex. LSD1-CoREST forms an elongated structure with a long stalk connecting the catalytic domain of LSD1 and the CoREST SANT2 domain. LSD1 recognizes a large segment of the H3 tail through a deep, negatively charged pocket at the active site and possibly a shallow groove on its surface. CoREST SANT2 interacts with DNA. Disruption of the SANT2-DNA interaction diminishes CoREST-dependent demethylation of nucleosomes by LSD1. The shape and dimension of LSD1-CoREST suggest its bivalent binding to nucleosomes, allowing efficient H3-K4 demethylation. This spatially separated, multivalent nucleosome binding mode may apply to other chromatin-modifying enzymes that generally contain multiple nucleosome binding modules. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Borek, D.]] | [[Category: Borek, D.]] | ||
| - | [[Category: Gocke, C | + | [[Category: Gocke, C B.]] |
[[Category: Luo, X.]] | [[Category: Luo, X.]] | ||
[[Category: Machius, M.]] | [[Category: Machius, M.]] | ||
[[Category: Otwinowski, Z.]] | [[Category: Otwinowski, Z.]] | ||
| - | [[Category: Tomchick, D | + | [[Category: Tomchick, D R.]] |
[[Category: Yang, M.]] | [[Category: Yang, M.]] | ||
[[Category: Yu, H.]] | [[Category: Yu, H.]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:56:35 2008'' |
Revision as of 15:56, 21 February 2008
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STRUCTURAL BASIS FOR COREST-DEPENDENT DEMETHYLATION OF NUCLEOSOMES BY THE HUMAN LSD1 HISTONE DEMETHYLASE
Overview
Histone methylation regulates diverse chromatin-templated processes, including transcription. Many transcriptional corepressor complexes contain lysine-specific demethylase 1 (LSD1) and CoREST that collaborate to demethylate mono- and dimethylated H3-K4 of nucleosomes. Here, we report the crystal structure of the LSD1-CoREST complex. LSD1-CoREST forms an elongated structure with a long stalk connecting the catalytic domain of LSD1 and the CoREST SANT2 domain. LSD1 recognizes a large segment of the H3 tail through a deep, negatively charged pocket at the active site and possibly a shallow groove on its surface. CoREST SANT2 interacts with DNA. Disruption of the SANT2-DNA interaction diminishes CoREST-dependent demethylation of nucleosomes by LSD1. The shape and dimension of LSD1-CoREST suggest its bivalent binding to nucleosomes, allowing efficient H3-K4 demethylation. This spatially separated, multivalent nucleosome binding mode may apply to other chromatin-modifying enzymes that generally contain multiple nucleosome binding modules.
About this Structure
2IW5 is a Protein complex structure of sequences from Homo sapiens with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase., Yang M, Gocke CB, Luo X, Borek D, Tomchick DR, Machius M, Otwinowski Z, Yu H, Mol Cell. 2006 Aug 4;23(3):377-87. PMID:16885027
Page seeded by OCA on Thu Feb 21 17:56:35 2008
Categories: Homo sapiens | Protein complex | Borek, D. | Gocke, C B. | Luo, X. | Machius, M. | Otwinowski, Z. | Tomchick, D R. | Yang, M. | Yu, H. | CL | FAD | GOL | NH4 | Alternative splicing | Chromatin demethylation | Chromatin regulator | Coiled coil | Corest | Fad | Histone demethylase | Host-virus interaction | Lsd1 | Nuclear protein | Nucleosomes | Oxidoreductase | Oxidoreductase/repressor complex | Phosphorylation | Repressor | Transcription | Transcription regulation
