2j3w
From Proteopedia
(New page: 200px<br /><applet load="2j3w" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j3w, resolution 2.1Å" /> '''THE CRYSTAL STRUCTURE...) |
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| - | [[Image:2j3w.jpg|left|200px]]<br /><applet load="2j3w" size=" | + | [[Image:2j3w.jpg|left|200px]]<br /><applet load="2j3w" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j3w, resolution 2.1Å" /> | caption="2j3w, resolution 2.1Å" /> | ||
'''THE CRYSTAL STRUCTURE OF THE BET3-TRS31-SEDLIN COMPLEX.'''<br /> | '''THE CRYSTAL STRUCTURE OF THE BET3-TRS31-SEDLIN COMPLEX.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Transport protein particle (TRAPP) I is a multisubunit vesicle tethering | + | Transport protein particle (TRAPP) I is a multisubunit vesicle tethering factor composed of seven subunits involved in ER-to-Golgi trafficking. The functional mechanism of the complex and how the subunits interact to form a functional unit are unknown. Here, we have used a multidisciplinary approach that includes X-ray crystallography, electron microscopy, biochemistry, and yeast genetics to elucidate the architecture of TRAPP I. The complex is organized through lateral juxtaposition of the subunits into a flat and elongated particle. We have also localized the site of guanine nucleotide exchange activity to a highly conserved surface encompassing several subunits. We propose that TRAPP I attaches to Golgi membranes with its large flat surface containing many highly conserved residues and forms a platform for protein-protein interactions. This study provides the most comprehensive view of a multisubunit vesicle tethering complex to date, based on which a model for the function of this complex, involving Rab1-GTP and long, coiled-coil tethers, is presented. |
==About this Structure== | ==About this Structure== | ||
| - | 2J3W is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 2J3W is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J3W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Kim, Y | + | [[Category: Kim, Y G.]] |
| - | [[Category: Oh, B | + | [[Category: Oh, B H.]] |
[[Category: endoplasmic reticulum]] | [[Category: endoplasmic reticulum]] | ||
[[Category: er-golgi transport]] | [[Category: er-golgi transport]] | ||
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[[Category: vesicle transport]] | [[Category: vesicle transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:58:56 2008'' |
Revision as of 15:58, 21 February 2008
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THE CRYSTAL STRUCTURE OF THE BET3-TRS31-SEDLIN COMPLEX.
Overview
Transport protein particle (TRAPP) I is a multisubunit vesicle tethering factor composed of seven subunits involved in ER-to-Golgi trafficking. The functional mechanism of the complex and how the subunits interact to form a functional unit are unknown. Here, we have used a multidisciplinary approach that includes X-ray crystallography, electron microscopy, biochemistry, and yeast genetics to elucidate the architecture of TRAPP I. The complex is organized through lateral juxtaposition of the subunits into a flat and elongated particle. We have also localized the site of guanine nucleotide exchange activity to a highly conserved surface encompassing several subunits. We propose that TRAPP I attaches to Golgi membranes with its large flat surface containing many highly conserved residues and forms a platform for protein-protein interactions. This study provides the most comprehensive view of a multisubunit vesicle tethering complex to date, based on which a model for the function of this complex, involving Rab1-GTP and long, coiled-coil tethers, is presented.
About this Structure
2J3W is a Protein complex structure of sequences from Danio rerio and Mus musculus. Full crystallographic information is available from OCA.
Reference
The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering., Kim YG, Raunser S, Munger C, Wagner J, Song YL, Cygler M, Walz T, Oh BH, Sacher M, Cell. 2006 Nov 17;127(4):817-30. PMID:17110339
Page seeded by OCA on Thu Feb 21 17:58:56 2008
