2j46
From Proteopedia
(New page: 200px<br /><applet load="2j46" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j46, resolution 1.135Å" /> '''WATER STRUCTURE OF ...) |
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| - | [[Image:2j46.jpg|left|200px]]<br /><applet load="2j46" size=" | + | [[Image:2j46.jpg|left|200px]]<br /><applet load="2j46" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j46, resolution 1.135Å" /> | caption="2j46, resolution 1.135Å" /> | ||
'''WATER STRUCTURE OF T. AQUATICUS FFH NG DOMAIN AT 1.1A RESOLUTION'''<br /> | '''WATER STRUCTURE OF T. AQUATICUS FFH NG DOMAIN AT 1.1A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Two new structures of the SRP GTPase Ffh have been determined at 1.1 A | + | Two new structures of the SRP GTPase Ffh have been determined at 1.1 A resolution and provide the basis for comparative examination of the extensive water structure of the apo conformation of these GTPases. A set of well defined water-binding positions have been identified in the active site of the two-domain ;NG' GTPase, as well as at two functionally important interfaces. The water hydrogen-bonding network accommodates alternate conformations of the protein side chains by undergoing local rearrangements and, in one case, illustrates binding of a solute molecule within the active site by displacement of water molecules without further disruption of the water-interaction network. A subset of the water positions are well defined in several lower resolution structures, including those of different nucleotide-binding states; these appear to function in maintaining the protein structure. Consistent arrangements of surface water between three different ultrahigh-resolution structures provide a framework for beginning to understand how local water structure contributes to protein-ligand and protein-protein binding in the SRP GTPases. |
==About this Structure== | ==About this Structure== | ||
| - | 2J46 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with CL, ACT, MN and MRD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2J46 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=MRD:'>MRD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J46 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
| - | [[Category: Freymann, D | + | [[Category: Freymann, D M.]] |
| - | [[Category: Ramirez, U | + | [[Category: Ramirez, U D.]] |
[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: water]] | [[Category: water]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:58:59 2008'' |
Revision as of 15:59, 21 February 2008
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WATER STRUCTURE OF T. AQUATICUS FFH NG DOMAIN AT 1.1A RESOLUTION
Overview
Two new structures of the SRP GTPase Ffh have been determined at 1.1 A resolution and provide the basis for comparative examination of the extensive water structure of the apo conformation of these GTPases. A set of well defined water-binding positions have been identified in the active site of the two-domain ;NG' GTPase, as well as at two functionally important interfaces. The water hydrogen-bonding network accommodates alternate conformations of the protein side chains by undergoing local rearrangements and, in one case, illustrates binding of a solute molecule within the active site by displacement of water molecules without further disruption of the water-interaction network. A subset of the water positions are well defined in several lower resolution structures, including those of different nucleotide-binding states; these appear to function in maintaining the protein structure. Consistent arrangements of surface water between three different ultrahigh-resolution structures provide a framework for beginning to understand how local water structure contributes to protein-ligand and protein-protein binding in the SRP GTPases.
About this Structure
2J46 is a Single protein structure of sequence from Thermus aquaticus with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Analysis of protein hydration in ultrahigh-resolution structures of the SRP GTPase Ffh., Ramirez UD, Freymann DM, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1520-34. Epub 2006, Nov 23. PMID:17139088
Page seeded by OCA on Thu Feb 21 17:58:59 2008
Categories: Single protein | Thermus aquaticus | Freymann, D M. | Ramirez, U D. | ACT | CL | MN | MRD | Ffh | Gtp-binding | Gtpase | Nucleotide-binding | Ribonucleoprotein | Rna-binding | Signal recognition particle | Srp | Water
