2j5o
From Proteopedia
(New page: 200px<br /><applet load="2j5o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j5o" /> '''PSEUDOMONAS AERUGINOSA FTSK GAMMA DOMAIN'''<...) |
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| - | [[Image:2j5o.gif|left|200px]]<br /><applet load="2j5o" size=" | + | [[Image:2j5o.gif|left|200px]]<br /><applet load="2j5o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j5o" /> | caption="2j5o" /> | ||
'''PSEUDOMONAS AERUGINOSA FTSK GAMMA DOMAIN'''<br /> | '''PSEUDOMONAS AERUGINOSA FTSK GAMMA DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The bacterial septum-located DNA translocase FtsK coordinates circular | + | The bacterial septum-located DNA translocase FtsK coordinates circular chromosome segregation with cell division. Rapid translocation of DNA by FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly replicated termini are brought together at the developing septum, thereby facilitating completion of chromosome segregation. Translocase functions reside in three domains, alpha, beta and gamma. FtsKalphabeta are necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsKgamma through its interaction with KOPS. By solving the FtsKgamma structure by NMR, we show that gamma is a winged-helix domain. NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. Mutated proteins with substitutions in the FtsKgamma DNA-recognition helix are impaired in DNA binding and KOPS recognition, yet remain competent in DNA translocation and XerCD-dif site-specific recombination, which facilitates the late stages of chromosome segregation. |
==About this Structure== | ==About this Structure== | ||
| - | 2J5O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http:// | + | 2J5O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Allen, M | + | [[Category: Allen, M D.]] |
[[Category: Arciszewska, L.]] | [[Category: Arciszewska, L.]] | ||
[[Category: Baker, R.]] | [[Category: Baker, R.]] | ||
[[Category: Bycroft, M.]] | [[Category: Bycroft, M.]] | ||
[[Category: Debekker, C.]] | [[Category: Debekker, C.]] | ||
| - | [[Category: Freund, S | + | [[Category: Freund, S M.]] |
[[Category: Lowe, J.]] | [[Category: Lowe, J.]] | ||
| - | [[Category: Sherratt, D | + | [[Category: Sherratt, D J.]] |
[[Category: Sivanathan, V.]] | [[Category: Sivanathan, V.]] | ||
[[Category: bacterial cell division]] | [[Category: bacterial cell division]] | ||
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[[Category: xercd]] | [[Category: xercd]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:59:29 2008'' |
Revision as of 15:59, 21 February 2008
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PSEUDOMONAS AERUGINOSA FTSK GAMMA DOMAIN
Overview
The bacterial septum-located DNA translocase FtsK coordinates circular chromosome segregation with cell division. Rapid translocation of DNA by FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly replicated termini are brought together at the developing septum, thereby facilitating completion of chromosome segregation. Translocase functions reside in three domains, alpha, beta and gamma. FtsKalphabeta are necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsKgamma through its interaction with KOPS. By solving the FtsKgamma structure by NMR, we show that gamma is a winged-helix domain. NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. Mutated proteins with substitutions in the FtsKgamma DNA-recognition helix are impaired in DNA binding and KOPS recognition, yet remain competent in DNA translocation and XerCD-dif site-specific recombination, which facilitates the late stages of chromosome segregation.
About this Structure
2J5O is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS., Sivanathan V, Allen MD, de Bekker C, Baker R, Arciszewska LK, Freund SM, Bycroft M, Lowe J, Sherratt DJ, Nat Struct Mol Biol. 2006 Nov;13(11):965-72. Epub 2006 Oct 22. PMID:17057717
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