2j7n
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | RNA silencing refers to a group of RNA-induced gene-silencing mechanisms | + | RNA silencing refers to a group of RNA-induced gene-silencing mechanisms that developed early in the eukaryotic lineage, probably for defence against pathogens and regulation of gene expression. In plants, protozoa, fungi, and nematodes, but apparently not insects and vertebrates, it involves a cell-encoded RNA-dependent RNA polymerase (cRdRP) that produces double-stranded RNA triggers from aberrant single-stranded RNA. We report the 2.3-A resolution crystal structure of QDE-1, a cRdRP from Neurospora crassa, and find that it forms a relatively compact dimeric molecule, each subunit of which comprises several domains with, at its core, a catalytic apparatus and protein fold strikingly similar to the catalytic core of the DNA-dependent RNA polymerases responsible for transcription. This evolutionary link between the two enzyme types suggests that aspects of RNA silencing in some organisms may recapitulate transcription/replication pathways functioning in the ancient RNA-based world. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Neurospora crassa]] | [[Category: Neurospora crassa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bamford, D | + | [[Category: Bamford, D H.]] |
| - | [[Category: Grimes, J | + | [[Category: Grimes, J M.]] |
| - | [[Category: Koivunen, M | + | [[Category: Koivunen, M R.L.]] |
| - | [[Category: Makeyev, E | + | [[Category: Makeyev, E V.]] |
| - | [[Category: Salgado, P | + | [[Category: Salgado, P S.]] |
| - | [[Category: Stuart, D | + | [[Category: Stuart, D I.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 27: | Line 27: | ||
[[Category: rnai response]] | [[Category: rnai response]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:00:11 2008'' |
Revision as of 16:00, 21 February 2008
|
STRUCTURE OF THE RNAI POLYMERASE FROM NEUROSPORA CRASSA
Overview
RNA silencing refers to a group of RNA-induced gene-silencing mechanisms that developed early in the eukaryotic lineage, probably for defence against pathogens and regulation of gene expression. In plants, protozoa, fungi, and nematodes, but apparently not insects and vertebrates, it involves a cell-encoded RNA-dependent RNA polymerase (cRdRP) that produces double-stranded RNA triggers from aberrant single-stranded RNA. We report the 2.3-A resolution crystal structure of QDE-1, a cRdRP from Neurospora crassa, and find that it forms a relatively compact dimeric molecule, each subunit of which comprises several domains with, at its core, a catalytic apparatus and protein fold strikingly similar to the catalytic core of the DNA-dependent RNA polymerases responsible for transcription. This evolutionary link between the two enzyme types suggests that aspects of RNA silencing in some organisms may recapitulate transcription/replication pathways functioning in the ancient RNA-based world.
About this Structure
2J7N is a Single protein structure of sequence from Neurospora crassa with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structure of an RNAi polymerase links RNA silencing and transcription., Salgado PS, Koivunen MR, Makeyev EV, Bamford DH, Stuart DI, Grimes JM, PLoS Biol. 2006 Dec;4(12):e434. PMID:17147473
Page seeded by OCA on Thu Feb 21 18:00:11 2008
