2j7w
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Dengue fever, a neglected emerging disease for which no vaccine or | + | Dengue fever, a neglected emerging disease for which no vaccine or antiviral agents exist at present, is caused by dengue virus, a member of the Flavivirus genus, which includes several important human pathogens, such as yellow fever and West Nile viruses. The NS5 protein from dengue virus is bifunctional and contains 900 amino acids. The S-adenosyl methionine transferase activity resides within its N-terminal domain, and residues 270 to 900 form the RNA-dependent RNA polymerase (RdRp) catalytic domain. Viral replication begins with the synthesis of minus-strand RNA from the dengue virus positive-strand RNA genome, which is subsequently used as a template for synthesizing additional plus-strand RNA genomes. This essential function for the production of new viral particles is catalyzed by the NS5 RdRp. Here we present a high-throughput in vitro assay partly recapitulating this activity and the crystallographic structure of an enzymatically active fragment of the dengue virus RdRp refined at 1.85-A resolution. The NS5 nuclear localization sequences, previously thought to fold into a separate domain, form an integral part of the polymerase subdomains. The structure also reveals the presence of two zinc ion binding motifs. In the absence of a template strand, a chain-terminating nucleoside analogue binds to the priming loop site. These results should inform and accelerate the structure-based design of antiviral compounds against dengue virus. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Canard, B.]] | [[Category: Canard, B.]] | ||
| - | [[Category: Chen, Y | + | [[Category: Chen, Y L.]] |
| - | [[Category: Egloff, M | + | [[Category: Egloff, M P.]] |
[[Category: Lescar, J.]] | [[Category: Lescar, J.]] | ||
[[Category: Malet, H.]] | [[Category: Malet, H.]] | ||
| - | [[Category: Vasudevan, S | + | [[Category: Vasudevan, S G.]] |
[[Category: Xu, T.]] | [[Category: Xu, T.]] | ||
| - | [[Category: Yap, T | + | [[Category: Yap, T L.]] |
[[Category: GTP]] | [[Category: GTP]] | ||
[[Category: PEG]] | [[Category: PEG]] | ||
| Line 31: | Line 31: | ||
[[Category: viral protein]] | [[Category: viral protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:00:15 2008'' |
Revision as of 16:00, 21 February 2008
|
DENGUE VIRUS NS5 RNA DEPENDENT RNA POLYMERASE DOMAIN COMPLEXED WITH 3'DGTP
Overview
Dengue fever, a neglected emerging disease for which no vaccine or antiviral agents exist at present, is caused by dengue virus, a member of the Flavivirus genus, which includes several important human pathogens, such as yellow fever and West Nile viruses. The NS5 protein from dengue virus is bifunctional and contains 900 amino acids. The S-adenosyl methionine transferase activity resides within its N-terminal domain, and residues 270 to 900 form the RNA-dependent RNA polymerase (RdRp) catalytic domain. Viral replication begins with the synthesis of minus-strand RNA from the dengue virus positive-strand RNA genome, which is subsequently used as a template for synthesizing additional plus-strand RNA genomes. This essential function for the production of new viral particles is catalyzed by the NS5 RdRp. Here we present a high-throughput in vitro assay partly recapitulating this activity and the crystallographic structure of an enzymatically active fragment of the dengue virus RdRp refined at 1.85-A resolution. The NS5 nuclear localization sequences, previously thought to fold into a separate domain, form an integral part of the polymerase subdomains. The structure also reveals the presence of two zinc ion binding motifs. In the absence of a template strand, a chain-terminating nucleoside analogue binds to the priming loop site. These results should inform and accelerate the structure-based design of antiviral compounds against dengue virus.
About this Structure
2J7W is a Single protein structure of sequence from Dengue virus with , and as ligands. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution., Yap TL, Xu T, Chen YL, Malet H, Egloff MP, Canard B, Vasudevan SG, Lescar J, J Virol. 2007 May;81(9):4753-65. Epub 2007 Feb 14. PMID:17301146
Page seeded by OCA on Thu Feb 21 18:00:15 2008
