2jek
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The X-ray crystal structure of the gene product encoded by open reading | + | The X-ray crystal structure of the gene product encoded by open reading frame Rv1873 of Mycobacterium tuberculosis has been determined by single isomorphous replacement with anomalous scattering (SIRAS) phasing techniques at 1.38 A resolution from monoclinic crystals with unit-cell parameters a = 33.44, b = 31.63, c = 53.19 A, beta = 90.8 degrees. The 16.2 kDa Rv1873 is a monomer that adopts a primarily alpha-helical fold with limited structural similarity to previously determined tertiary structures. It has been annotated as a conserved hypothetical protein of unknown function and is classified by the Clusters of Orthologous Groups (COG) database as belonging to COG5579. The three-dimensional structure of the Rv1873 gene product reveals limited similarity to a repeated motif that is found in a variety of other proteins. While not a novel fold, it serves as a model for orthologues predicted to be related by sequence and it is hoped that knowledge of the structure of Rv1873 will aid in determining a possible function for this protein. |
==About this Structure== | ==About this Structure== | ||
| - | 2JEK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 2JEK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 2D2Y. Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEK OCA]. |
==Reference== | ==Reference== | ||
| - | The molecular structure of Rv1873, a conserved hypothetical protein from Mycobacterium tuberculosis, at 1.38 A resolution., Garen CR, Cherney MM, Bergmann EM, James MN, Acta | + | The molecular structure of Rv1873, a conserved hypothetical protein from Mycobacterium tuberculosis, at 1.38 A resolution., Garen CR, Cherney MM, Bergmann EM, James MN, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1201-5. Epub 2006 Nov 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17142896 17142896] |
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bergmann, E | + | [[Category: Bergmann, E M.]] |
| - | [[Category: Cherney, M | + | [[Category: Cherney, M M.]] |
| - | [[Category: Garen, C | + | [[Category: Garen, C R.]] |
| - | [[Category: James, M | + | [[Category: James, M N.G.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: unknown function]] | [[Category: unknown function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:02:22 2008'' |
Revision as of 16:02, 21 February 2008
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CRYSTAL STRUCTURE OF THE CONSERVED HYPOTHETICAL PROTEIN RV1873 FROM MYCOBACTERIUM TUBERCULOSIS AT 1.38 A
Overview
The X-ray crystal structure of the gene product encoded by open reading frame Rv1873 of Mycobacterium tuberculosis has been determined by single isomorphous replacement with anomalous scattering (SIRAS) phasing techniques at 1.38 A resolution from monoclinic crystals with unit-cell parameters a = 33.44, b = 31.63, c = 53.19 A, beta = 90.8 degrees. The 16.2 kDa Rv1873 is a monomer that adopts a primarily alpha-helical fold with limited structural similarity to previously determined tertiary structures. It has been annotated as a conserved hypothetical protein of unknown function and is classified by the Clusters of Orthologous Groups (COG) database as belonging to COG5579. The three-dimensional structure of the Rv1873 gene product reveals limited similarity to a repeated motif that is found in a variety of other proteins. While not a novel fold, it serves as a model for orthologues predicted to be related by sequence and it is hoped that knowledge of the structure of Rv1873 will aid in determining a possible function for this protein.
About this Structure
2JEK is a Single protein structure of sequence from Mycobacterium tuberculosis with and as ligands. This structure supersedes the now removed PDB entry 2D2Y. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The molecular structure of Rv1873, a conserved hypothetical protein from Mycobacterium tuberculosis, at 1.38 A resolution., Garen CR, Cherney MM, Bergmann EM, James MN, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1201-5. Epub 2006 Nov 30. PMID:17142896
Page seeded by OCA on Thu Feb 21 18:02:22 2008
Categories: Mycobacterium tuberculosis | Single protein | Bergmann, E M. | Cherney, M M. | Garen, C R. | James, M N.G. | TBSGC, TB Structural Genomics Consortium. | GOL | SO4 | Hypothetical protein | Protein structure initiative | Psi | Right-handed superhelix | Structural genomics | Tb structural genomics consortium | Tbsgc | Unknown function
