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2jm3
From Proteopedia
(New page: 200px<br /><applet load="2jm3" size="350" color="white" frame="true" align="right" spinBox="true" caption="2jm3" /> '''Solution structure of the THAP domain from C...) |
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==Overview== | ==Overview== | ||
| - | The THAP (Thanatos-associated protein) domain is a recently discovered | + | The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Crossley, M.]] | [[Category: Crossley, M.]] | ||
| - | [[Category: Liew, C | + | [[Category: Liew, C K.]] |
| - | [[Category: Mackay, J | + | [[Category: Mackay, J P.]] |
| - | [[Category: Nicholas, H | + | [[Category: Nicholas, H R.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: domain]] | [[Category: domain]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:03:53 2008'' |
Revision as of 16:03, 21 February 2008
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Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)
Overview
The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers.
About this Structure
2JM3 is a Single protein structure of sequence from Caenorhabditis elegans with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP)., Liew CK, Crossley M, Mackay JP, Nicholas HR, J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978
Page seeded by OCA on Thu Feb 21 18:03:53 2008
