2jmc
From Proteopedia
(New page: 200px<br /><applet load="2jmc" size="350" color="white" frame="true" align="right" spinBox="true" caption="2jmc" /> '''Chimer between Spc-SH3 and P41'''<br /> ==O...) |
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==Overview== | ==Overview== | ||
| - | Here we present the high-resolution NMR structure of a chimera (SPCp41) | + | Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bruix, M.]] | [[Category: Bruix, M.]] | ||
| - | [[Category: Candel, A | + | [[Category: Candel, A M.]] |
[[Category: Conejero-Lara, F.]] | [[Category: Conejero-Lara, F.]] | ||
| - | [[Category: Martinez, J | + | [[Category: Martinez, J C.]] |
| - | [[Category: Nuland, N | + | [[Category: Nuland, N A.J van.]] |
[[Category: chimer]] | [[Category: chimer]] | ||
[[Category: p41]] | [[Category: p41]] | ||
[[Category: spc-sh3]] | [[Category: spc-sh3]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:03:57 2008'' |
Revision as of 16:03, 21 February 2008
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Chimer between Spc-SH3 and P41
Overview
Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.
About this Structure
2JMC is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex., Candel AM, Conejero-Lara F, Martinez JC, van Nuland NA, Bruix M, FEBS Lett. 2007 Feb 20;581(4):687-92. Epub 2007 Jan 22. PMID:17275816
Page seeded by OCA on Thu Feb 21 18:03:57 2008
